Proteintech

FGL1 Polyclonal Antibody

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FGL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 16000-1-AP) (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FGL1 Antibody in Western Blot (WB) — Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

FGL1 Antibody in Immunoprecipitation (IP) — IP result of anti-FGL1 (IP:16000-1-AP, 4ug; Detection:16000-1-AP 1:500) with HeLa cells lysate 2000ug.

Product Details

16000-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FGL1 Fusion Protein Ag8581 (1-312 aa encoded by BC007047)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MAKVFSFIL VTTALTMGRE ISALEDCAQE QMRLRAQVRL LETRVKQQQV KIKQLLQENE VQFLDKGDEN TVIDLGSKRQ YADCSEIFND GYKLSGFYKI KPLQSPAEFS VYCDMSDGGG WTVIQRRSDG SENFNRGWKD YENGFGNFVQ KHGEYWLGNK NLHFLTTQED YTLKIDLADF EKNSRYAQYK NFKVGDEKNF YELNIGEYSG TAGDSLAGNF HPEVQWWASH QRMKFSTWDR DHDNYEGNCA EEDQSGWWFN RCHSANLNGV YYSGPYTAKT DNGIVWYTWH GWWYSLKSVV MKIRPNDFIP NVI (1-312 aa encoded by BC007047)

Target Information

Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting fibrinogen-like protein 1 enhances immunotherapy in hepatocellular carcinoma.

The Journal of clinical investigation

Lin M,He J,Zhang X,Sun X,Dong W,Zhang R,Xu Y,Lv L

Mon May 01 00:00:00 EDT 2023

Targeting fibrinogen-like protein 1 enhances immunotherapy in hepatocellular carcinoma.

The Journal of clinical investigation

Lin M,He J,Zhang X,Sun X,Dong W,Zhang R,Xu Y,Lv L

Mon May 01 00:00:00 EDT 2023

Fibrinogen-like protein 1 promotes liver-resident memory T-cell exhaustion in hepatocellular carcinoma.

Frontiers in immunology

Yang C,Qian Q,Zhao Y,Huang B,Chen R,Gong Q,Ji H,Wang C,Xia L,You Z,Zhang J,Chen X

Thu Apr 06 00:00:00 EDT 2023

Dual immunological and proliferative regulation of immune checkpoint FGL1 in lung adenocarcinoma: The pivotal role of the YY1-FGL1-MYH9 axis.

Frontiers in immunology

Tang XY,Xiong YL,Zhao YB,Yang J,Shi AP,Zheng KF,Liu YJ,Shu C,Jiang T,Ma N,Zhao JB

Mon Oct 24 00:00:00 EDT 2022

Dual immunological and proliferative regulation of immune checkpoint FGL1 in lung adenocarcinoma: The pivotal role of the YY1-FGL1-MYH9 axis.

Frontiers in immunology

Tang XY,Xiong YL,Zhao YB,Yang J,Shi AP,Zheng KF,Liu YJ,Shu C,Jiang T,Ma N,Zhao JB

Mon Oct 24 00:00:00 EDT 2022

The downregulation of fibrinogen-like protein 1 inhibits the proliferation of lung adenocarcinoma via regulating MYC-target genes.

Translational lung cancer research

Tang XY,Xiong YL,Shi AP,Sun Y,Han Q,Lv Y,Shi XG,Frattini M,Malhotra J,Zheng KF,Liu YJ,Jiang T,Ma N,Zhao JB

Tue Mar 01 00:00:00 EST 2022

The downregulation of fibrinogen-like protein 1 inhibits the proliferation of lung adenocarcinoma via regulating MYC-target genes.

Translational lung cancer research

Tang XY,Xiong YL,Shi AP,Sun Y,Han Q,Lv Y,Shi XG,Frattini M,Malhotra J,Zheng KF,Liu YJ,Jiang T,Ma N,Zhao JB

Tue Mar 01 00:00:00 EST 2022

The downregulation of fibrinogen-like protein 1 inhibits the proliferation of lung adenocarcinoma via regulating MYC-target genes.

Translational lung cancer research

Tang XY,Xiong YL,Shi AP,Sun Y,Han Q,Lv Y,Shi XG,Frattini M,Malhotra J,Zheng KF,Liu YJ,Jiang T,Ma N,Zhao JB

Tue Mar 01 00:00:00 EST 2022

Proteomics identifies a novel role of fibrinogen-like protein 1 in Crohn's disease.

World journal of gastroenterology

Sun XL,Qiao LC,Gong J,Wen K,Xu ZZ,Yang BL

Tue Sep 21 00:00:00 EDT 2021

Proteomics identifies a novel role of fibrinogen-like protein 1 in Crohn's disease.

World journal of gastroenterology

Sun XL,Qiao LC,Gong J,Wen K,Xu ZZ,Yang BL

Tue Sep 21 00:00:00 EDT 2021

Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells.

International journal of molecular sciences

Son Y,Shin NR,Kim SH,Park SC,Lee HJ

Wed May 19 00:00:00 EDT 2021

Eicosapentaenoic Acid Inhibits KRAS Mutant Pancreatic Cancer Cell Growth by Suppressing Hepassocin Expression and STAT3 Phosphorylation.

Biomolecules

Chiu CF,Hsu MI,Yeh HY,Park JM,Shen YS,Tung TH,Huang JJ,Wu HT,Huang SY

Tue Mar 02 00:00:00 EST 2021

Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles.

Journal of nanobiotechnology

Gong C,Yu X,Zhang W,Han L,Wang R,Wang Y,Gao S,Yuan Y

Thu Feb 25 00:00:00 EST 2021

Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles.

Journal of nanobiotechnology

Gong C,Yu X,Zhang W,Han L,Wang R,Wang Y,Gao S,Yuan Y

Thu Feb 25 00:00:00 EST 2021

Oxysophocarpine suppresses hepatocellular carcinoma growth and sensitizes the therapeutic blockade of anti-Lag-3 via reducing FGL1 expression.

Cancer medicine

Wang J,Wei W,Tang Q,Lu L,Luo Z,Li W,Lu Y,Pu J

Thu Oct 01 00:00:00 EDT 2020

Role of placental fibrinogen-like protein 1 in gestational diabetes.

Translational research : the journal of laboratory and clinical medicine

Kang L,Li HY,Ou HY,Wu P,Wang SH,Chang CJ,Lin SY,Wu CL,Wu HT

Wed Apr 01 00:00:00 EDT 2020

Role of placental fibrinogen-like protein 1 in gestational diabetes.

Translational research : the journal of laboratory and clinical medicine

Kang L,Li HY,Ou HY,Wu P,Wang SH,Chang CJ,Lin SY,Wu CL,Wu HT

Wed Apr 01 00:00:00 EDT 2020

Targeting fibrinogen-like protein 1 is a novel therapeutic strategy to combat obesity.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wu HT,Chen SC,Fan KC,Kuo CH,Lin SY,Wang SH,Chang CJ,Li HY

Sat Feb 01 00:00:00 EST 2020

The co-expression characteristics of LAG3 and PD-1 on the T cells of patients with breast cancer reveal a new therapeutic strategy.

International immunopharmacology

Du H,Yi Z,Wang L,Li Z,Niu B,Ren G

Wed Jan 01 00:00:00 EST 2020

Plasma Fibrinogen-Like 1 as a Potential Biomarker for Radiation-Induced Liver Injury.

Cells

Han NK,Jung MG,Jeong YJ,Son Y,Han SC,Park S,Lim YB,Lee YJ,Kim SH,Park SC,Lee HJ

Fri Sep 06 00:00:00 EDT 2019

Unsaturated Fatty Acids Increase the Expression of Hepassocin through a Signal Transducer and Activator of Transcription 3-Dependent Pathway in HepG2 Cells.

Lipids

Cheng KP,Ou HY,Hung HC,Li CH,Fan KC,Wu JS,Wu HT,Chang CJ

Sat Sep 01 00:00:00 EDT 2018

Unsaturated Fatty Acids Increase the Expression of Hepassocin through a Signal Transducer and Activator of Transcription 3-Dependent Pathway in HepG2 Cells.

Lipids

Cheng KP,Ou HY,Hung HC,Li CH,Fan KC,Wu JS,Wu HT,Chang CJ

Sat Sep 01 00:00:00 EDT 2018

The Hepatic Protection Effects of Hepassocin in Hyperglycemic Crisis.

The Journal of clinical endocrinology and metabolism

Ou HY,Wu HT,Lin CH,Du YF,Hu CY,Hung HC,Wu P,Li HY,Wang SH,Chang CJ

Sat Jul 01 00:00:00 EDT 2017

A novel hepatokine, HFREP1, plays a crucial role in the development of insulin resistance and type 2 diabetes.

Diabetologia

Wu HT,Ou HY,Hung HC,Su YC,Lu FH,Wu JS,Yang YC,Wu CL,Chang CJ

Mon Aug 01 00:00:00 EDT 2016

A novel hepatokine, HFREP1, plays a crucial role in the development of insulin resistance and type 2 diabetes.

Diabetologia

Wu HT,Ou HY,Hung HC,Su YC,Lu FH,Wu JS,Yang YC,Wu CL,Chang CJ

Mon Aug 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: fibrinogen-like 1; Fibrinogen-like protein 1; Fibrinogen-related protein 1; fibronigen-like protein 1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HP-041; HPS; LFIRE-1; Liver fibrinogen-related protein 1; Liver fibrinogen-related protein-1; MFIRE-1; Mfrep-1; MGC12455; OTTHUMP00000122468

Gene Aliases: FGL1; Frep1; HFREP1; HP-041; LFIRE-1; LFIRE1; Mfire1

UniProt ID: (Human) Q08830, (Rat) Q5M8C6, (Mouse) Q71KU9

Entrez Gene ID: (Human) 2267, (Rat) 246186, (Mouse) 234199

Molecular Function: intercellular signal molecule