Immunofluorescence analysis of FHIT Polyclonal Antibody was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FHIT Rabbit Polyclonal Antibody at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full length human FHIT fusion protein with GST (glutathione amino transferase)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Fhit regulates EMT targets through an EGFR/Src/ERK/Slug signaling axis in human bronchial cells.
71-9000 was used in western blot to investigate the mechanism of fragile histidine triad-mediated regulation of epithelial-mesenchymal transition.
|Joannes A,Grelet S,Duca L,Gilles C,Kileztky C,Dalstein V,Birembaut P,Polette M,Nawrocki-Raby B||Molecular cancer research : MCR (12:775)||2014|
The apoptotic pathway triggered by the Fhit protein in lung cancer cell lines is not affected by Bcl-2 or Bcl-x(L) overexpression.
71-9000 was used in western blot to elucidate how fragile histidine triad proteins suppress the development of cancer
|Roz L,Andriani F,Ferreira CG,Giaccone G,Sozzi G||Oncogene (23:9102)||2004|
Reduced FHIT expression is associated with mismatch repair deficient and high CpG island methylator phenotype colorectal cancer.
71-9000 was used in immunohistochemistry to study the fragile histidine triad gene in microsatellite instable, microsatellite stable, and CpG island methylator phenotypes of colorectal cancer subtypes.
|Al-Temaimi RA,Jacob S,Al-Ali W,Thomas DA,Al-Mulla F||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (61:627)||2013|
|Human||Not Cited||Differential protein immunoexpression profiles in appendiceal mucinous neoplasms: a special reference to classification and predictive factors.||Yoon SO,Kim BH,Lee HS,Kang GH,Kim WH,Kim YA,Kim JE,Chang MS||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (22:1102)||2009|
|Human||Not Cited||Protein expression profiling identifies subclasses of breast cancer and predicts prognosis.||Jacquemier J,Ginestier C,Rougemont J,Bardou VJ,Charafe-Jauffret E,Geneix J,Adélaïde J,Koki A,Houvenaeghel G,Hassoun J,Maraninchi D,Viens P,Birnbaum D,Bertucci F||Cancer research (65:767)||2005|
|Mouse||1:200||Loss of fragile histidine triad protein in human hepatocellular carcinoma.||Zhao P,Song X,Nin YY,Lu YL,Li XH||World journal of gastroenterology (9:1216)||2003|
|Human||Not Cited||Alterations of the fragile histidine triad gene, FHIT, and its encoded products contribute to testicular germ cell tumorigenesis.||Kraggerud SM,Aman P,Holm R,Stenwig AE,Fosså SD,Nesland JM,Lothe RA||Cancer research (62:512)||2002|