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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length human FHIT protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is normal breast tissue.
Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Promoter methylation of tumor suppressor genes in pre-neoplastic lesions; potential marker of disease recurrence.
PA1-37690 was used in immunohistochemistry - paraffin section to determine if epigenetic alterations predict colon cancer recurrence
|Rengucci C,De Maio G,Casadei Gardini A,Zucca M,Scarpi E,Zingaretti C,Foschi G,Tumedei MM,Molinari C,Saragoni L,Puccetti M,Amadori D,Zoli W,Calistri D||Journal of experimental and clinical cancer research : CR (33:null)||2014|
AP3A hydrolase; AP3Aase; Bis(5'-adenosyl)-triphosphatase; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; FRA3B; Fragile histidine triad protein
AP3Aase; FHIT; FRA3B