Proteintech

FHL1 Polyclonal Antibody

FHL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FHL1 Antibody (10991-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human ovary tumor using 10991-1-AP (FHL1 antibody) at dilution of 1:100 (under 10x lens).

FHL1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10991-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Human, Mouse, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FHL1 Fusion Protein Ag1448 (1-280 aa encoded by BC010998)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This is a rabbit polyclonal antibody raised against full length of human FHL1.

Immunogen sequence: MAEKFDCHY CRDPLQGKKY VQKDGHHCCL KCFDKFCANT CVECRKPIGA DSKEVHYKNR FWHDTCFRCA KCLHPLANET FVAKDNKILC NKCTTREDSP KCKGCFKAIV AGDQNVEYKG TVWHKDCFTC SNCKQVIGTG SFFPKGEDFY CVTCHETKFA KHCVKCNKAI TSGGITYQDQ PWHADCFVCV TCSKKLAGQR FTAVEDQYYC VDCYKNFVAK KCAGCKNPIT GFGKGSSVVA YEGQSWHDYC FHCKKCSVNL ANKRFVFHQE QVYCPDCAKK L (1-280 aa encoded by BC010998)

Target Information

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Cell biology and toxicology

Luo Q,Pan Y,Fu Q,Zhang X,Zhou S,Yu P,Tian H,Liu P,Chen S,Zhang H,Qin T

Sun Oct 01 00:00:00 UTC 2023

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Cell biology and toxicology

Luo Q,Pan Y,Fu Q,Zhang X,Zhou S,Yu P,Tian H,Liu P,Chen S,Zhang H,Qin T

Sun Oct 01 00:00:00 UTC 2023

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Cell biology and toxicology

Luo Q,Pan Y,Fu Q,Zhang X,Zhou S,Yu P,Tian H,Liu P,Chen S,Zhang H,Qin T

Sun Oct 01 00:00:00 UTC 2023

Identification of novel FHL1 mutations associated with X-linked scapuloperoneal myopathy in unrelated Chinese patients.

Journal of human genetics

Lin Y,Ban R,Qiao L,Chen J,Liu M,Liu J,Shi Q

Sat Jul 01 00:00:00 UTC 2023

Identification of novel FHL1 mutations associated with X-linked scapuloperoneal myopathy in unrelated Chinese patients.

Journal of human genetics

Lin Y,Ban R,Qiao L,Chen J,Liu M,Liu J,Shi Q

Sat Jul 01 00:00:00 UTC 2023

Disruption of the CCDC43-FHL1 interaction triggers apoptosis in gastric cancer cells.

Experimental cell research

Chen Y,Pei M,Li J,Wang Z,Liu S,Xiang L,Zhang J,Hong L,Lin J,Dai W,Xiao Y,Hu H,Tang W,Liu G,Yang Q,Lin Z,Jiang X,Wang Y,Wu X,Guo Z,Wang J

Wed Jun 01 00:00:00 UTC 2022

Disruption of the CCDC43-FHL1 interaction triggers apoptosis in gastric cancer cells.

Experimental cell research

Chen Y,Pei M,Li J,Wang Z,Liu S,Xiang L,Zhang J,Hong L,Lin J,Dai W,Xiao Y,Hu H,Tang W,Liu G,Yang Q,Lin Z,Jiang X,Wang Y,Wu X,Guo Z,Wang J

Wed Jun 01 00:00:00 UTC 2022

Disruption of the CCDC43-FHL1 interaction triggers apoptosis in gastric cancer cells.

Experimental cell research

Chen Y,Pei M,Li J,Wang Z,Liu S,Xiang L,Zhang J,Hong L,Lin J,Dai W,Xiao Y,Hu H,Tang W,Liu G,Yang Q,Lin Z,Jiang X,Wang Y,Wu X,Guo Z,Wang J

Wed Jun 01 00:00:00 UTC 2022

Inhibition of Jumonji demethylases reprograms severe dilated cardiomyopathy and prolongs survival.

The Journal of biological chemistry

Tran TA,Zhang QJ,Wang L,Gonzales C,Girard L,May H,Gillette T,Liu ZP,Martinez ED

Tue Feb 01 00:00:00 UTC 2022

Downregulation of FHL1 protein in glioma inhibits tumor growth through PI3K/AKT signaling.

Oncology letters

Li SZ,Hu YY,Zhao JL,Zang J,Fei Z,Han H,Qin HY

Mon Jun 01 00:00:00 UTC 2020

Structural and Functional Characterization of Host FHL1 Protein Interaction with Hypervariable Domain of Chikungunya Virus nsP3 Protein.

Journal of virology

Lukash T,Agback T,Dominguez F,Shiliaev N,Meshram C,Frolova EI,Agback P,Frolov I

Wed Dec 09 00:00:00 UTC 2020

Structural and Functional Characterization of Host FHL1 Protein Interaction with Hypervariable Domain of Chikungunya Virus nsP3 Protein.

Journal of virology

Lukash T,Agback T,Dominguez F,Shiliaev N,Meshram C,Frolova EI,Agback P,Frolov I

Wed Dec 09 00:00:00 UTC 2020

Journal of human genetics

Hu Z,Zhu Y,Liu X,Zhang W,Liu J,Wu S,Xiao J,Yuan Y,Wang Z

Sun Sep 01 00:00:00 UTC 2019

Hypoxia-induced alterations in the lung ubiquitin proteasome system during pulmonary hypertension pathogenesis.

Pulmonary circulation

Wade BE,Zhao J,Ma J,Hart CM,Sutliff RL

Tue Sep 29 00:00:00 UTC 2020

Hypoxia-induced alterations in the lung ubiquitin proteasome system during pulmonary hypertension pathogenesis.

Pulmonary circulation

Wade BE,Zhao J,Ma J,Hart CM,Sutliff RL

Tue Sep 29 00:00:00 UTC 2020

Src-mediated phosphorylation converts FHL1 from tumor suppressor to tumor promoter.

The Journal of cell biology

Wang X,Wei X,Yuan Y,Sun Q,Zhan J,Zhang J,Tang Y,Li F,Ding L,Ye Q,Zhang H

Mon Apr 02 00:00:00 UTC 2018

A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.

Nature communications

Xu X,Fan Z,Liang C,Li L,Wang L,Liang Y,Wu J,Chang S,Yan Z,Lv Z,Fu J,Liu Y,Jin S,Wang T,Hong T,Dong Y,Ding L,Cheng L,Liu R,Fu S,Jiao S,Ye Q

Tue Jan 17 00:00:00 UTC 2017

A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.

Nature communications

Xu X,Fan Z,Liang C,Li L,Wang L,Liang Y,Wu J,Chang S,Yan Z,Lv Z,Fu J,Liu Y,Jin S,Wang T,Hong T,Dong Y,Ding L,Cheng L,Liu R,Fu S,Jiao S,Ye Q

Tue Jan 17 00:00:00 UTC 2017

A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.

Nature communications

Xu X,Fan Z,Liang C,Li L,Wang L,Liang Y,Wu J,Chang S,Yan Z,Lv Z,Fu J,Liu Y,Jin S,Wang T,Hong T,Dong Y,Ding L,Cheng L,Liu R,Fu S,Jiao S,Ye Q

Tue Jan 17 00:00:00 UTC 2017

A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.

Nature communications

Xu X,Fan Z,Liang C,Li L,Wang L,Liang Y,Wu J,Chang S,Yan Z,Lv Z,Fu J,Liu Y,Jin S,Wang T,Hong T,Dong Y,Ding L,Cheng L,Liu R,Fu S,Jiao S,Ye Q

Tue Jan 17 00:00:00 UTC 2017

Role of Zebrafish fhl1A in Satellite Cell and Skeletal Muscle Development.

Current molecular medicine

Chen F,Yuan W,Mo X,Zhuang J,Wang Y,Chen J,Jiang Z,Zhu X,Zeng Q,Wan Y,Li F,Shi Y,Cao L,Fan X,Luo S,Ye X,Chen Y,Dai G,Gao J,Wang X,Xie H,Zhu P,Li Y,Wu X

Tue Jun 11 00:00:00 UTC 2019

Browning of human adipocytes requires KLF11 and reprogramming of PPARγ superenhancers.

Genes & development

Loft A,Forss I,Siersbæk MS,Schmidt SF,Larsen AS,Madsen JG,Pisani DF,Nielsen R,Aagaard MM,Mathison A,Neville MJ,Urrutia R,Karpe F,Amri EZ,Mandrup S

Thu Jan 01 00:00:00 UTC 2015

Browning of human adipocytes requires KLF11 and reprogramming of PPARγ superenhancers.

Genes & development

Loft A,Forss I,Siersbæk MS,Schmidt SF,Larsen AS,Madsen JG,Pisani DF,Nielsen R,Aagaard MM,Mathison A,Neville MJ,Urrutia R,Karpe F,Amri EZ,Mandrup S

Thu Jan 01 00:00:00 UTC 2015

The LIM domain protein FHL1C interacts with tight junction protein ZO-1 contributing to the epithelial-mesenchymal transition (EMT) of a breast adenocarcinoma cell line.

Gene

Zhao JL,Liang SQ,Fu W,Zhu BK,Li SZ,Han H,Qin HY

Sun Jun 01 00:00:00 UTC 2014

The LIM domain protein FHL1C interacts with tight junction protein ZO-1 contributing to the epithelial-mesenchymal transition (EMT) of a breast adenocarcinoma cell line.

Gene

Zhao JL,Liang SQ,Fu W,Zhu BK,Li SZ,Han H,Qin HY

Sun Jun 01 00:00:00 UTC 2014

MiR-410 is overexpressed in liver and colorectal tumors and enhances tumor cell growth by silencing FHL1 via a direct/indirect mechanism.

PloS one

Wang Y,Fu J,Jiang M,Zhang X,Cheng L,Xu X,Fan Z,Zhang J,Ye Q,Song H

Wed Jun 10 00:00:00 UTC 2015

MiR-410 is overexpressed in liver and colorectal tumors and enhances tumor cell growth by silencing FHL1 via a direct/indirect mechanism.

PloS one

Wang Y,Fu J,Jiang M,Zhang X,Cheng L,Xu X,Fan Z,Zhang J,Ye Q,Song H

Wed Jun 10 00:00:00 UTC 2015

FHL1C induces apoptosis in Notch1-dependent T-ALL cells through an interaction with RBP-J.

BMC cancer

Fu W,Wang K,Zhao JL,Yu HC,Li SZ,Lin Y,Liang L,Huang SY,Liang YM,Han H,Qin HY

Sun Jun 22 00:00:00 UTC 2014

FHL1C induces apoptosis in Notch1-dependent T-ALL cells through an interaction with RBP-J.

BMC cancer

Fu W,Wang K,Zhao JL,Yu HC,Li SZ,Lin Y,Liang L,Huang SY,Liang YM,Han H,Qin HY

Sun Jun 22 00:00:00 UTC 2014

Evaluation of protein expression pattern of stanniocalcin 2, insulin-like growth factor-binding protein 7, inhibin beta A and four and a half LIM domains 1 in esophageal squamous cell carcinoma.

Cancer biomarkers : section A of Disease markers

Kashyap MK,Pawar HA,Keerthikumar S,Sharma J,Goel R,Mahmood R,Kumar MV,Kumar KV,Pandey A,Kumar RV,Prasad TS,Harsha HC

Fri Jul 12 00:00:00 UTC 2013

Evaluation of protein expression pattern of stanniocalcin 2, insulin-like growth factor-binding protein 7, inhibin beta A and four and a half LIM domains 1 in esophageal squamous cell carcinoma.

Cancer biomarkers : section A of Disease markers

Kashyap MK,Pawar HA,Keerthikumar S,Sharma J,Goel R,Mahmood R,Kumar MV,Kumar KV,Pandey A,Kumar RV,Prasad TS,Harsha HC

Fri Jul 12 00:00:00 UTC 2013

The LIM protein fhlA is essential for heart chamber development in zebrafish embryos.

Current molecular medicine

Xie H,Fan X,Tang X,Wan Y,Chen F,Wang X,Wang Y,Li Y,Tang M,Liu D,Jiang Z,Liu X,Yuan W,Li G,Ye X,Zhou J,Mo X,Deng Y,Wu X

Mon Jul 01 00:00:00 UTC 2013

The LIM protein fhlA is essential for heart chamber development in zebrafish embryos.

Current molecular medicine

Xie H,Fan X,Tang X,Wan Y,Chen F,Wang X,Wang Y,Li Y,Tang M,Liu D,Jiang Z,Liu X,Yuan W,Li G,Ye X,Zhou J,Mo X,Deng Y,Wu X

Mon Jul 01 00:00:00 UTC 2013

Downregulation and growth inhibitory role of FHL1 in lung cancer.

International journal of cancer

Niu C,Liang C,Guo J,Cheng L,Zhang H,Qin X,Zhang Q,Ding L,Yuan B,Xu X,Li J,Lin J,Ye Q

Fri Jun 01 00:00:00 UTC 2012

Downregulation and growth inhibitory role of FHL1 in lung cancer.

International journal of cancer

Niu C,Liang C,Guo J,Cheng L,Zhang H,Qin X,Zhang Q,Ding L,Yuan B,Xu X,Li J,Lin J,Ye Q

Fri Jun 01 00:00:00 UTC 2012

Downregulation and growth inhibitory role of FHL1 in lung cancer.

International journal of cancer

Niu C,Liang C,Guo J,Cheng L,Zhang H,Qin X,Zhang Q,Ding L,Yuan B,Xu X,Li J,Lin J,Ye Q

Fri Jun 01 00:00:00 UTC 2012

Downregulation and growth inhibitory role of FHL1 in lung cancer.

International journal of cancer

Niu C,Liang C,Guo J,Cheng L,Zhang H,Qin X,Zhang Q,Ding L,Yuan B,Xu X,Li J,Lin J,Ye Q

Fri Jun 01 00:00:00 UTC 2012

FHL family members suppress vascular endothelial growth factor expression through blockade of dimerization of HIF1α and HIF1β.

IUBMB life

Lin J,Qin X,Zhu Z,Mu J,Zhu L,Wu K,Jiao H,Xu X,Ye Q

Thu Nov 01 00:00:00 UTC 2012

FHL family members suppress vascular endothelial growth factor expression through blockade of dimerization of HIF1α and HIF1β.

IUBMB life

Lin J,Qin X,Zhu Z,Mu J,Zhu L,Wu K,Jiao H,Xu X,Ye Q

Thu Nov 01 00:00:00 UTC 2012

FHL1 interacts with oestrogen receptors and regulates breast cancer cell growth.

Journal of cellular and molecular medicine

Ding L,Niu C,Zheng Y,Xiong Z,Liu Y,Lin J,Sun H,Huang K,Yang W,Li X,Ye Q

Sat Jan 01 00:00:00 UTC 2011

FHL1 interacts with oestrogen receptors and regulates breast cancer cell growth.

Journal of cellular and molecular medicine

Ding L,Niu C,Zheng Y,Xiong Z,Liu Y,Lin J,Sun H,Huang K,Yang W,Li X,Ye Q

Sat Jan 01 00:00:00 UTC 2011

MAL/MRTF-A controls migration of non-invasive cells by upregulation of cytoskeleton-associated proteins.

Journal of cell science

Leitner L,Shaposhnikov D,Mengel A,Descot A,Julien S,Hoffmann R,Posern G

Thu Dec 15 00:00:00 UTC 2011

MAL/MRTF-A controls migration of non-invasive cells by upregulation of cytoskeleton-associated proteins.

Journal of cell science

Leitner L,Shaposhnikov D,Mengel A,Descot A,Julien S,Hoffmann R,Posern G

Thu Dec 15 00:00:00 UTC 2011

MAL/MRTF-A controls migration of non-invasive cells by upregulation of cytoskeleton-associated proteins.

Journal of cell science

Leitner L,Shaposhnikov D,Mengel A,Descot A,Julien S,Hoffmann R,Posern G

Thu Dec 15 00:00:00 UTC 2011

CpG hypermethylation of human four-and-a-half LIM domains 1 contributes to migration and invasion activity of human bladder cancer.

International journal of molecular medicine

Matsumoto M,Kawakami K,Enokida H,Toki K,Matsuda R,Chiyomaru T,Nishiyama K,Kawahara K,Seki N,Nakagawa M

Sun Aug 01 00:00:00 UTC 2010

CpG hypermethylation of human four-and-a-half LIM domains 1 contributes to migration and invasion activity of human bladder cancer.

International journal of molecular medicine

Matsumoto M,Kawakami K,Enokida H,Toki K,Matsuda R,Chiyomaru T,Nishiyama K,Kawahara K,Seki N,Nakagawa M

Sun Aug 01 00:00:00 UTC 2010

Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.