Proteintech

FHL1 Polyclonal Antibody

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FHL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor using 10991-1-AP (FHL1 antibody) at dilution of 1:100 (under 10x lens).

FHL1 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FHL1 Antibody in Immunoprecipitation (IP) — IP result of anti-FHL1 (IP:10991-1-AP, 3ug; Detection:10991-1-AP 1:700) with mouse skeletal muscle tissue lysate 3600ug.

Product Details

10991-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2400	View 14 publications 14 publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 2 publications 2 publications
ChIP assay (ChIP)	Assay-dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat, Zebrafish
Published species	Human, Mouse, Zebrafish
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FHL1 Fusion Protein Ag1448 (1-280 aa encoded by BC010998) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This is a rabbit polyclonal antibody raised against full length of human FHL1.

Immunogen sequence: MAEKFDCHY CRDPLQGKKY VQKDGHHCCL KCFDKFCANT CVECRKPIGA DSKEVHYKNR FWHDTCFRCA KCLHPLANET FVAKDNKILC NKCTTREDSP KCKGCFKAIV AGDQNVEYKG TVWHKDCFTC SNCKQVIGTG SFFPKGEDFY CVTCHETKFA KHCVKCNKAI TSGGITYQDQ PWHADCFVCV TCSKKLAGQR FTAVEDQYYC VDCYKNFVAK KCAGCKNPIT GFGKGSSVVA YEGQSWHDYC FHCKKCSVNL ANKRFVFHQE QVYCPDCAKK L (1-280 aa encoded by BC010998)

Target Information

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hypoxia-induced alterations in the lung ubiquitin proteasome system during pulmonary hypertension pathogenesis.

Pulmonary circulation

Wade BE,Zhao J,Ma J,Hart CM,Sutliff RL

Tue Sep 29 00:00:00 EDT 2020

A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.

Nature communications

Xu X,Fan Z,Liang C,Li L,Wang L,Liang Y,Wu J,Chang S,Yan Z,Lv Z,Fu J,Liu Y,Jin S,Wang T,Hong T,Dong Y,Ding L,Cheng L,Liu R,Fu S,Jiao S,Ye Q

Tue Jan 17 00:00:00 EST 2017

A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.

Nature communications

Xu X,Fan Z,Liang C,Li L,Wang L,Liang Y,Wu J,Chang S,Yan Z,Lv Z,Fu J,Liu Y,Jin S,Wang T,Hong T,Dong Y,Ding L,Cheng L,Liu R,Fu S,Jiao S,Ye Q

Tue Jan 17 00:00:00 EST 2017

MiR-410 is overexpressed in liver and colorectal tumors and enhances tumor cell growth by silencing FHL1 via a direct/indirect mechanism.

PloS one

Wang Y,Fu J,Jiang M,Zhang X,Cheng L,Xu X,Fan Z,Zhang J,Ye Q,Song H

Wed Jun 10 00:00:00 EDT 2015

Browning of human adipocytes requires KLF11 and reprogramming of PPARγ superenhancers.

Genes & development

Loft A,Forss I,Siersbæk MS,Schmidt SF,Larsen AS,Madsen JG,Pisani DF,Nielsen R,Aagaard MM,Mathison A,Neville MJ,Urrutia R,Karpe F,Amri EZ,Mandrup S

Thu Jan 01 00:00:00 EST 2015

FHL1C induces apoptosis in Notch1-dependent T-ALL cells through an interaction with RBP-J.

BMC cancer

Fu W,Wang K,Zhao JL,Yu HC,Li SZ,Lin Y,Liang L,Huang SY,Liang YM,Han H,Qin HY

Sun Jun 22 00:00:00 EDT 2014

The LIM domain protein FHL1C interacts with tight junction protein ZO-1 contributing to the epithelial-mesenchymal transition (EMT) of a breast adenocarcinoma cell line.

Gene

Zhao JL,Liang SQ,Fu W,Zhu BK,Li SZ,Han H,Qin HY

Sun Jun 01 00:00:00 EDT 2014

Evaluation of protein expression pattern of stanniocalcin 2, insulin-like growth factor-binding protein 7, inhibin beta A and four and a half LIM domains 1 in esophageal squamous cell carcinoma.

Cancer biomarkers : section A of Disease markers

Kashyap MK,Pawar HA,Keerthikumar S,Sharma J,Goel R,Mahmood R,Kumar MV,Kumar KV,Pandey A,Kumar RV,Prasad TS,Harsha HC

Fri Jul 12 00:00:00 EDT 2013

The LIM protein fhlA is essential for heart chamber development in zebrafish embryos.

Current molecular medicine

Xie H,Fan X,Tang X,Wan Y,Chen F,Wang X,Wang Y,Li Y,Tang M,Liu D,Jiang Z,Liu X,Yuan W,Li G,Ye X,Zhou J,Mo X,Deng Y,Wu X

Mon Jul 01 00:00:00 EDT 2013

FHL family members suppress vascular endothelial growth factor expression through blockade of dimerization of HIF1α and HIF1β.

IUBMB life

Lin J,Qin X,Zhu Z,Mu J,Zhu L,Wu K,Jiao H,Xu X,Ye Q

Thu Nov 01 00:00:00 EDT 2012

Downregulation and growth inhibitory role of FHL1 in lung cancer.

International journal of cancer

Niu C,Liang C,Guo J,Cheng L,Zhang H,Qin X,Zhang Q,Ding L,Yuan B,Xu X,Li J,Lin J,Ye Q

Fri Jun 01 00:00:00 EDT 2012

Downregulation and growth inhibitory role of FHL1 in lung cancer.

International journal of cancer

Niu C,Liang C,Guo J,Cheng L,Zhang H,Qin X,Zhang Q,Ding L,Yuan B,Xu X,Li J,Lin J,Ye Q

Fri Jun 01 00:00:00 EDT 2012

MAL/MRTF-A controls migration of non-invasive cells by upregulation of cytoskeleton-associated proteins.

Journal of cell science

Leitner L,Shaposhnikov D,Mengel A,Descot A,Julien S,Hoffmann R,Posern G

Thu Dec 15 00:00:00 EST 2011

MAL/MRTF-A controls migration of non-invasive cells by upregulation of cytoskeleton-associated proteins.

Journal of cell science

Leitner L,Shaposhnikov D,Mengel A,Descot A,Julien S,Hoffmann R,Posern G

Thu Dec 15 00:00:00 EST 2011

FHL1 interacts with oestrogen receptors and regulates breast cancer cell growth.

Journal of cellular and molecular medicine

Ding L,Niu C,Zheng Y,Xiong Z,Liu Y,Lin J,Sun H,Huang K,Yang W,Li X,Ye Q

Sat Jan 01 00:00:00 EST 2011

CpG hypermethylation of human four-and-a-half LIM domains 1 contributes to migration and invasion activity of human bladder cancer.

International journal of molecular medicine

Matsumoto M,Kawakami K,Enokida H,Toki K,Matsuda R,Chiyomaru T,Nishiyama K,Kawahara K,Seki N,Nakagawa M

Sun Aug 01 00:00:00 EDT 2010

Four and a half LIM domains 1 (FHL1) and receptor interacting protein of 140kDa (RIP140) interact and cooperate in estrogen signaling.

The international journal of biochemistry & cell biology

Lin J,Ding L,Jin R,Zhang H,Cheng L,Qin X,Chai J,Ye Q

Wed Jul 01 00:00:00 EDT 2009

Four and a half LIM domains 1 (FHL1) and receptor interacting protein of 140kDa (RIP140) interact and cooperate in estrogen signaling.

The international journal of biochemistry & cell biology

Lin J,Ding L,Jin R,Zhang H,Cheng L,Qin X,Chai J,Ye Q

Wed Jul 01 00:00:00 EDT 2009

Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.

The Journal of clinical investigation

Ding L,Wang Z,Yan J,Yang X,Liu A,Qiu W,Zhu J,Han J,Zhang H,Lin J,Cheng L,Qin X,Niu C,Yuan B,Wang X,Zhu C,Zhou Y,Li J,Song H,Huang C,Ye Q

Sun Feb 01 00:00:00 EST 2009

Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.

The Journal of clinical investigation

Ding L,Wang Z,Yan J,Yang X,Liu A,Qiu W,Zhu J,Han J,Zhang H,Lin J,Cheng L,Qin X,Niu C,Yuan B,Wang X,Zhu C,Zhou Y,Li J,Song H,Huang C,Ye Q

Sun Feb 01 00:00:00 EST 2009

Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.

The Journal of clinical investigation

Ding L,Wang Z,Yan J,Yang X,Liu A,Qiu W,Zhu J,Han J,Zhang H,Lin J,Cheng L,Qin X,Niu C,Yuan B,Wang X,Zhu C,Zhou Y,Li J,Song H,Huang C,Ye Q

Sun Feb 01 00:00:00 EST 2009

Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.

The Journal of clinical investigation

Ding L,Wang Z,Yan J,Yang X,Liu A,Qiu W,Zhu J,Han J,Zhang H,Lin J,Cheng L,Qin X,Niu C,Yuan B,Wang X,Zhu C,Zhou Y,Li J,Song H,Huang C,Ye Q

Sun Feb 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: bA535K18.1; FHL; FHL-1; four and a half LIM domains a; Four and a half LIM domains protein 1; four-and-a-half Lin11, Isl-1 and Mec-3 domains 1; KYO-T; KyoT; LIM protein SLIMMER; MGC111107; RAM14-1; RBP-associated molecule 14-1; RP11-535K18.1; Skeletal muscle LIM-protein 1; SLIM

Gene Aliases: cb1039; fhl; FHL-1; FHL1; FHL1A; FHL1B; fhla; FLH1A; KYOT; RAM14-1; RBMX1A; RBMX1B; SLIM; SLIM-1; SLIM1; SLIMMER; XMPMA; zgc:92025

UniProt ID: (Human) Q13642, (Rat) Q9WUH4, (Mouse) P97447

Entrez Gene ID: (Human) 2273, (Rat) 25177, (Mouse) 14199, (Zebrafish) 399646, (Zebrafish) 387528

Molecular Function: transcription cofactor