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FIH1 Polyclonal Antibody
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Product Details
BS-4201R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human FIH1/HIF1AN, amino acids 251-349.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: co-repressor; DKFZp762F1811; Factor inhibiting HIF-1; factor inhibiting HIF1; FIH 1; FIH-1; FLJ20615; FLJ22027; hypoxia inducible factor 1 alpha subunit inhibitor; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase; unnamed protein product
Gene Aliases: 2310046M24Rik; A830014H24Rik; FIH; FIH1; HIF1AN
UniProt ID: (Human) Q9NWT6, (Mouse) Q8BLR9
Entrez Gene ID: (Human) 55662, (Rat) 309434, (Mouse) 319594
transcription corepressor activity
Notch binding
protein binding
ferrous iron binding
zinc ion binding
oxidoreductase activity
oxygen sensor activity
carboxylic acid binding
peptidyl-histidine dioxygenase activity
peptidyl-asparagine 3-dioxygenase activity
protein homodimerization activity
metal ion binding
NF-kappaB binding
dioxygenase activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
peptidyl-aspartic acid 3-dioxygenase activity
ankyrin repeat binding
molecular_function
iron ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
cofactor binding
biological_process
peptidyl-histidine hydroxylation
peptidyl-aspartic acid hydroxylation
peptidyl-asparagine hydroxylation
positive regulation of myoblast differentiation
negative regulation of Notch signaling pathway
oxidation-reduction process
negative regulation of transcription from RNA polymerase II promoter in response to hypoxia
negative regulation of transcription from RNA polymerase II promoter
regulation of signal transduction
regulation of vascular endothelial growth factor receptor signaling pathway
regulation of developmental process
positive regulation of vasculogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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