Proteintech

FIS1 Polyclonal Antibody

Product Image Gallery

FIS1 Antibody in Immunocytochemistry (ICC/IF) — IF result of anti-FIS1 (10956-1-AP,1:100) with Hepa1-6 cell by Dr. Steven Eugene Smith. Mitochondrion outer membrane (Green) Stain.

FIS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer using 10956-1-AP (FIS1 antibody) at dilution of 1:50 (under 10x lens).

FIS1 Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FIS1 Antibody in Immunoprecipitation (IP) — IP result of anti-FIS1 (IP:10956-1-AP, 3ug; Detection:10956-1-AP 1:500) with HeLa cells lysate 3000ug.

Product Details

10956-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:16,000	View 48 publications 48 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:100	-
Immunocytochemistry (ICC/IF)	1:50-1:200	View 11 publications 11 publications
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Pig, Rat
Published species	Human, Mouse, Rat, Rodent
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FIS1 Fusion Protein Ag1409 (1-152 aa encoded by BC009428) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates.

Immunogen sequence: MEAVLNELV SVEDLLKFEK KFQSEKAAGS VSKSTQFEYA WCLVRSKYND DIRKGIVLLE ELLPKGSKEE QRDYVFYLAV GNYRLKEYEK ALKYVRGLLQ TEPQNNQAKE LERLIDKAMK KDGLVGMAIV GGMALGVAGL AGLIGLAVSK SKS (1-152 aa encoded by BC009428)

Target Information

Fis1, a 17KDa integral membrane protein, is a novel member of mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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A novel Drp1 inhibitor diminishes aberrant mitochondrial fission and neurotoxicity.

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Biochimica et biophysica acta

Manczak M,Sesaki H,Kageyama Y,Reddy PH

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Fri Jun 01 00:00:00 EDT 2012

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Impaired mitochondrial dynamics and abnormal interaction of amyloid beta with mitochondrial protein Drp1 in neurons from patients with Alzheimer's disease: implications for neuronal damage.

Human molecular genetics

Manczak M,Calkins MJ,Reddy PH

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Abnormal mitochondrial dynamics, mitochondrial loss and mutant huntingtin oligomers in Huntington's disease: implications for selective neuronal damage.

Human molecular genetics

Shirendeb U,Reddy AP,Manczak M,Calkins MJ,Mao P,Tagle DA,Reddy PH

Fri Apr 01 00:00:00 EDT 2011

Abnormal mitochondrial dynamics, mitochondrial loss and mutant huntingtin oligomers in Huntington's disease: implications for selective neuronal damage.

Human molecular genetics

Shirendeb U,Reddy AP,Manczak M,Calkins MJ,Mao P,Tagle DA,Reddy PH

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Mitochondria-targeted antioxidants protect against amyloid-beta toxicity in Alzheimer's disease neurons.

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Bioinformatics

Protein Aliases: FIS1 homolog; fis1 {ECO:0000250|UniProtKB:Q9Y3D6}; fission 1 (mitochondrial outer membrane) homolog; H_NH0132A01.6; hFis1; Mitochondrial fission 1 protein; mitochondrial fission molecule; rFis1; tetratricopeptide repeat domain 11; Tetratricopeptide repeat protein 11; TPR repeat protein 11

Gene Aliases: 2010003O14Rik; CGI-135; FIS1; TTC11

UniProt ID: (Human) Q9Y3D6, (Rat) P84817, (Mouse) Q9CQ92

Entrez Gene ID: (Pig) 100622909, (Human) 51024, (Rat) 288584, (Mouse) 66437

Function(s)