Invitrogen

FIS1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

7 Published Figures

11 References

View all (18) FIS1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

FIS1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

FIS1 Antibody (PA5-22142) in ICC/IF

FIS1 Polyclonal Antibody detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FIS1 protein stained by FIS1 Polyclonal Antibody (Product # PA5-22142) diluted at 1:2,000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Product Image Gallery

FIS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — TTC11 antibody detects TTC11 protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. TTC11 antibody (Product # PA5-22142) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

FIS1 Antibody in Western Blot (WB) — Western Blot using FIS1 Polyclonal Antibody (Product # PA5-22142). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 15% SDS PAGE. FIS1 Polyclonal Antibody (Product # PA5-22142) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

FIS1 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 5 UCP2 deletion increased the levels of mitochondrial fission-related proteins in hyperglycemic mice after ischemia/reperfusion. Detections of mitochondrial fission-related proteins by immunohistochemistry and Western blotting (n=6, per group). (A, B) Representative photomicrographs for Fis1 and Drp1. Scale bar=50 mum. (C, D) Quantification of Fis1 and Drp1 immunointensity. E: Representative Western blots for Fis1 and Drp1. (F, G) Semi-quantification of Fis1 and Drp1 protein bands. Data are shown as mean +- SD. For values in F and G, the values in WT+NG sham group were converted to 100 and percent changes were presented for other groups relative to the WT+NG sham. Delta p <0.05 vs. respective sham-operated controls, # p <0.05 vs. normoglycemic and * p <0.05 vs. WT counterpart at an identical reperfusion stage.

FIS1 Antibody in Immunohistochemistry (IHC) — Figure 8 Mitochondrial fusion-related proteins (Opa1 and Mfn2) and fission-related proteins (Drp1, Fis1) co-localized with neurons in UCP2 -/- mice following ischemic stroke. Double immunostaining of Drp1, Fis1, Opa1 and Mfn2 with GFAP (astrocyte marker) and NeuN (neuron marker) were performed in UCP2 -/- mice brain sections 1 day after reperfusion (n=6, each group). Scale bar =25 mum.

FIS1 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. HeLa cells were transfected with FIS1 shRNA and decrease in signal intensity was observed in Western blot application using FIS1 Polyclonal Antibody (Product # PA5-22142). {KD}

Product Details

PA5-22142

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:2,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full-length human FIS1 recombinant protein

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11152577

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Raji, Mouse brain, mouse liver, rat brain.

Predicted reactivity: Mouse (97%), Rat (97%), Rhesus Monkey (100%), Bovine (97%).

Target Information

Fis1, a 17KDa integral membrane protein, is a novel member of mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Multi-Targeting Intranasal Nanoformulation as a Therapeutic for Alzheimer's Disease.

Biomolecules

Fihurka O,Wang Y,Hong Y,Lin X,Shen N,Yang H,Brown B,Mommer M,Zieneldien T,Li Y,Kim J,Li M,Cai J,Zhou Q,Cao C

PA5-22142 was used in Western Blotting to indicate that MIT treatment significantly decreased the tau phosphorylation implicated in tau toxicity (p 0.05) and the expression of CKMT1 associated with mitochondrial dysfunction.

Wed Jan 25 00:00:00 EST 2023

Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells.

Molecular medicine (Cambridge, Mass.)

Quiring L,Walter B,Lohaus N,Schwan D,Rech A,Dlugos A,Rauen U

Published figure using FIS1 polyclonal antibody (Product # PA5-22142) in Western Blot

Mon Jan 31 00:00:00 EST 2022

A-Kinase Anchor Protein 1 deficiency causes mitochondrial dysfunction in mouse model of hyperoxia induced acute lung injury.

Frontiers in pharmacology

Soundararajan R,Hernández-Cuervo H,Stearns TM,Griswold AJ,Patil SS,Fukumoto J,Narala VR,Galam L,Lockey R,Kolliputi N

Published figure using FIS1 polyclonal antibody (Product # PA5-22142) in Western Blot

Fri Oct 21 00:00:00 EDT 2022

A-Kinase Anchor Protein 1 deficiency causes mitochondrial dysfunction in mouse model of hyperoxia induced acute lung injury.

Frontiers in pharmacology

Soundararajan R,Hernández-Cuervo H,Stearns TM,Griswold AJ,Patil SS,Fukumoto J,Narala VR,Galam L,Lockey R,Kolliputi N

Published figure using FIS1 polyclonal antibody (Product # PA5-22142) in Western Blot

Fri Oct 21 00:00:00 EDT 2022

Changes in Drp1 Function and Mitochondrial Morphology Are Associated with the α-Synuclein Pathology in a Transgenic Mouse Model of Parkinson's Disease.

Cells

Portz P,Lee MK

Published figure using FIS1 polyclonal antibody (Product # PA5-22142) in Western Blot

Tue Apr 13 00:00:00 EDT 2021

Changes in Drp1 Function and Mitochondrial Morphology Are Associated with the α-Synuclein Pathology in a Transgenic Mouse Model of Parkinson's Disease.

Cells

Portz P,Lee MK

Published figure using FIS1 polyclonal antibody (Product # PA5-22142) in Western Blot

Tue Apr 13 00:00:00 EDT 2021

All-Trans Retinoic Acid Increases DRP1 Levels and Promotes Mitochondrial Fission.

Cells

Chidipi B,Shah SI,Reiser M,Kanithi M,Garces A,Cha BJ,Ullah G,Noujaim SF

PA5-22142 was used in Western Blot to investigate the direct effects of the micronutrient retinoid all-trans retinoic acid (ATRA) on the mitochondrial structure.

Fri May 14 00:00:00 EDT 2021

Azoramide protects iPSC-derived dopaminergic neurons with PLA2G6 D331Y mutation through restoring ER function and CREB signaling.

Cell death & disease

Ke M,Chong CM,Zeng H,Huang M,Huang Z,Zhang K,Cen X,Lu JH,Yao X,Qin D,Su H

Published figure using FIS1 polyclonal antibody (Product # PA5-22142) in Western Blot

Tue Feb 18 00:00:00 EST 2020

Azoramide protects iPSC-derived dopaminergic neurons with PLA2G6 D331Y mutation through restoring ER function and CREB signaling.

Cell death & disease

Ke M,Chong CM,Zeng H,Huang M,Huang Z,Zhang K,Cen X,Lu JH,Yao X,Qin D,Su H

Published figure using FIS1 polyclonal antibody (Product # PA5-22142) in Western Blot

Tue Feb 18 00:00:00 EST 2020

Inhibition of chymotrypsin-like activity of the proteasome by ixazomib prevents mitochondrial dysfunction during myocardial ischemia.

PloS one

Sánchez G,Chalmers S,Ahumada X,Montecinos L,Olmedo I,Eisner V,Riveros A,Kogan MJ,Lavandero S,Pedrozo Z,Donoso P

Published figure using FIS1 polyclonal antibody (Product # PA5-22142) in Western Blot

Fri Aug 14 00:00:00 EDT 2020

Inhibition of chymotrypsin-like activity of the proteasome by ixazomib prevents mitochondrial dysfunction during myocardial ischemia.

PloS one

Sánchez G,Chalmers S,Ahumada X,Montecinos L,Olmedo I,Eisner V,Riveros A,Kogan MJ,Lavandero S,Pedrozo Z,Donoso P

Published figure using FIS1 polyclonal antibody (Product # PA5-22142) in Western Blot

Fri Aug 14 00:00:00 EDT 2020

Deletion of Mitochondrial Uncoupling Protein 2 Exacerbates Mitochondrial Damage in Mice Subjected to Cerebral Ischemia and Reperfusion Injury under both Normo- and Hyperglycemic Conditions.

International journal of biological sciences

He M,Ma Y,Wang R,Zhang J,Jing L,Li PA

Published figure using FIS1 polyclonal antibody (Product # PA5-22142) in Immunohistochemistry

Mon Apr 04 00:00:00 EDT 2022

Deletion of Mitochondrial Uncoupling Protein 2 Exacerbates Mitochondrial Damage in Mice Subjected to Cerebral Ischemia and Reperfusion Injury under both Normo- and Hyperglycemic Conditions.

International journal of biological sciences

He M,Ma Y,Wang R,Zhang J,Jing L,Li PA

Published figure using FIS1 polyclonal antibody (Product # PA5-22142) in Immunohistochemistry

Mon Apr 04 00:00:00 EDT 2022

Deletion of Mitochondrial Uncoupling Protein 2 Exacerbates Mitochondrial Damage in Mice Subjected to Cerebral Ischemia and Reperfusion Injury under both Normo- and Hyperglycemic Conditions.

International journal of biological sciences

He M,Ma Y,Wang R,Zhang J,Jing L,Li PA

Published figure using FIS1 polyclonal antibody (Product # PA5-22142) in Immunohistochemistry

Mon Apr 04 00:00:00 EDT 2022

Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.

Cell host & microbe

Jean Beltran PM,Cook KC,Hashimoto Y,Galitzine C,Murray LA,Vitek O,Cristea IM

PA5-22142 was used in Immunocytochemistry-immunoflourescence to discover if the enveloped viruses human cytomegalovirus (HCMV) and herpes simplex virus type 1 (HSV-1) induce the biogenesis of and unique morphological changes to peroxisomes to support their replication.

Wed Oct 10 00:00:00 EDT 2018

Ubiquitin-mediated regulation of the E3 ligase GP78 by MGRN1 in trans affects mitochondrial homeostasis.

Journal of cell science

Mukherjee R,Chakrabarti O

PA5-22142 was used in western blot to determine how mitochondrial homeostasis is affected by ubiquitin-mediated regulation of E3 ligase GP78 by MGRN1 in trans

Mon Feb 15 00:00:00 EST 2016

Bioinformatics

Protein Aliases: FIS1 homolog; fis1 {ECO:0000250|UniProtKB:Q9Y3D6}; fission 1 (mitochondrial outer membrane) homolog; H_NH0132A01.6; hFis1; Mitochondrial fission 1 protein; mitochondrial fission molecule; rFis1; tetratricopeptide repeat domain 11; Tetratricopeptide repeat protein 11; TPR repeat protein 11

Gene Aliases: 2010003O14Rik; CGI-135; FIS1; TTC11

UniProt ID: (Human) Q9Y3D6, (Rat) P84817, (Mouse) Q9CQ92

Entrez Gene ID: (Human) 51024, (Rat) 288584, (Mouse) 66437

Function(s)