Immunofluorescent analysis of FIS1 showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min and stained using a FIS1 polyclonal antibody (Product # PA5-22142) diluted at 1:2000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 152 of TTC11 (Uniprot ID#Q9Y3D6)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22142 targets FIS1 in ICC, IF, IHC (P), and WB applications and shows reactivity with Human, rat, and mouse samples.
The PA5-22142 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 152 (Q9Y3D6) of TTC11.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.