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Invitrogen
FITC Polyclonal Antibody
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FIGURE: 1 / 11
FITC Antibody (71-1900) in ICC/IF
Nature communications 2018 -
Fig. 2 GLP-1R agonist trafficking in MIN6B1-SNAP-GLP-1R cells. a Schematic for GLP-1R internalization and recycling measurements by FACS after labeling with cleavable SNAP-Surface probe. b Agonist-induced GLP-1R internalization in MIN6B1-SNAP-GLP-1R cells, n = 3, two-way randomized block ANOVA with Dunnett's test vs. exendin-4. c GLP-1R recycling in MIN6B1-SNAP-GLP-1R cells 30 min after an initial 15 min ag... View More
Product Details
71-1900
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical,
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
FITC
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody was purified from serum of rabbit hyper-immunized with FITC conjugated to KLH. The antibody was purified by antigen-specific matrix. The antibody reacts with FITC but not the carrier protein. Useful as a secondary antibody in a wide range of immunoassays including: ELISA, tissue and cell staining, immunoprecipitation, and western blotting. The protein concentration is determined based on an E (1%) = 14 at 280 nm.
Target Information
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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