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Invitrogen
FITC Polyclonal Antibody
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FIGURE: 1 / 1
FITC Antibody (PA1-26793) in IP
Nature communications 2022 -
Fig. 2 KP1 competes with TGF-beta1 for TbetaR2 binding. a , b Co-immunoprecipitation (Co-IP) demonstrated that KP1 bound to TbetaR2. NRK-49F cell lysates (500 ug) and FITC-KP1 (10 ug) were immunoprecipitated (IP) with the anti-TbetaR2 antibody at 4 degC overnight, followed by immunoblotted (IB) for FITC and TbetaR2, respectively ( a ). In the reciprocal experiment, mixtures of cell lysates and FITC-KP1 were... View More
Product Details
PA1-26793
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Tag
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Fluorescein conjugated to Goat IgG.
Conjugate
Form
Liquid
Concentration
1.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-26793 detects Fluorescein.
Target Information
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Fluorescein
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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