Immunohistochemistry was performed on cancer biopsies of deparaffinized human Colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing FKBP12 (Product #PA1-026A) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Pig, Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal residues G(1) V Q V E T I S P G D G R(13) of human FKBP12.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||2 ug/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-026A detects FK506 binding protein 12 kDa (FKBP12) in human and mouse tissues.
PA1-026A has been successfully used in Western blot, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 12 kDa protein representing FKBP12 from rat heart homogenate.
The PA1-026A immunogen is a synthetic peptide corresponding to the N-terminal residues G(1) V Q V E T I S P G D G R(13) of human FKBP12. PA1-026A immunizing peptide (Cat. # PEP-012) is available for use in neutralization and control experiments.
Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins.
FKBP12:FK506 complexes inhibit calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase which blocks T-cell activation by preventing lymphokine gene transcription. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. In interactions with members of the TGF beta family type I receptors, FKBP12 has been shown to exert an inhibitory effect on the signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Vaccination with Necroptotic Cancer Cells Induces Efficient Anti-tumor Immunity.
PA1-026A was used in western blot to analyze induction of efficient anti-tumor immunity by vaccination with necroptotic cancer cells
|Aaes TL,Kaczmarek A,Delvaeye T,De Craene B,De Koker S,Heyndrickx L,Delrue I,Taminau J,Wiernicki B,De Groote P,Garg AD,Leybaert L,Grooten J,Bertrand MJ,Agostinis P,Berx G,Declercq W,Vandenabeele P,Krysko DV||Cell reports (15:274)||2016|
The composition of EphB2 clusters determines the strength in the cellular repulsion response.
PA1-026A was used in western blot to study the relationship between the strength of the cellular repulsion response and the composition of EphB2 clusters
|Schaupp A,Sabet O,Dudanova I,Ponserre M,Bastiaens P,Klein R||The Journal of cell biology (204:409)||2014|
mTOR pathway and Ca²¿ stores mobilization in aged smooth muscle cells.
PA1-026A was used in western blot to study the involvement of the mTor pathway in age-related changes in smooth muscle cell Ca(2+) mobilization
|Martín-Cano FE,Camello-Almaraz C,Hernandez D,Pozo MJ,Camello PJ||Aging (5:339)||2013|
Transgenic analysis of the role of FKBP12.6 in cardiac function and intracellular calcium release.
PA1-026A was used in western blot to investigate the role of FK506 binding protein12.6 FKBP12 in intracellular calcium release and heart function
|Liu Y,Chen H,Ji G,Li B,Mohler PJ,Zhu Z,Yong W,Chen Z,Xu X,Xin H,Shou W||Assay and drug development technologies (9:620)||2011|
Relieving autophagy and 4EBP1 from rapamycin resistance.
PA1-026A was used in western blot to investigate the regulation of autophagy by rapamycin in different cell lines
|Nyfeler B,Bergman P,Triantafellow E,Wilson CJ,Zhu Y,Radetich B,Finan PM,Klionsky DJ,Murphy LO||Molecular and cellular biology (31:2867)||2011|
Localization of the dantrolene-binding sequence near the FK506-binding protein-binding site in the three-dimensional structure of the ryanodine receptor.
PA1-026A was used in western blot to investigate the interaction between dantrolene and ryanodine receptor
|Wang R,Zhong X,Meng X,Koop A,Tian X,Jones PP,Fruen BR,Wagenknecht T,Liu Z,Chen SR||The Journal of biological chemistry (286:12202)||2011|
Prevention of ventricular arrhythmia and calcium dysregulation in a catecholaminergic polymorphic ventricular tachycardia mouse model carrying calsequestrin-2 mutation.
PA1-026A was used in western blot to identify therapeutic targets in ventricular arrhythmia and calcium dysregulation
|Alcalai R,Wakimoto H,Arad M,Planer D,Konno T,Wang L,Seidman JG,Seidman CE,Berul CI||Journal of cardiovascular electrophysiology (22:316)||2011|
Organelle-specific, rapid induction of molecular activities and membrane tethering.
PA1-026A was used in western blot to develop a new method of targeting proteins to individual intracellular organelles
|Komatsu T,Kukelyansky I,McCaffery JM,Ueno T,Varela LC,Inoue T||Nature methods (7:206)||2010|
Inhibition of FK506 binding proteins reduces alpha-synuclein aggregation and Parkinson's disease-like pathology.
PA1-026A was used in western blot to study the role of FKBPs in aggregation and apoptosis
|Gerard M,Deleersnijder A,Daniëls V,Schreurs S,Munck S,Reumers V,Pottel H,Engelborghs Y,Van den Haute C,Taymans JM,Debyser Z,Baekelandt V||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:2454)||2010|
Expression levels of RyR1 and RyR3 control resting free Ca2+ in skeletal muscle.
PA1-026A was used in western blot to investigate the effect of the transient expression of ryanodine receptor (RyR) type 3 and 1 on calcium homeostasis in skeletal muscle
|Perez CF,López JR,Allen PD||American journal of physiology. Cell physiology (288:C640)||2005|
Gene silencing in androgen-responsive prostate cancer cells from the tissue-specific prostate-specific antigen promoter.
PA1-026A was used in western blot to study the gene silencing in androgen-responsive prostate cancer cells with a prostate-specific antigen promoter.
|Song J,Pang S,Lu Y,Yokoyama KK,Zheng JY,Chiu R||Cancer research (64:7661)||2004|
Cyclophilin A is required for retinoic acid-induced neuronal differentiation in p19 cells.
PA1-026A was used in western blot to investigate the role of cyclophilin A in the retinoic acid-induced p19 cell differentiation.
|Song J,Lu YC,Yokoyama K,Rossi J,Chiu R||The Journal of biological chemistry (279:24414)||2004|
The 12 kDa FK506-binding protein, FKBP12, modulates the Ca(2+)-flux properties of the type-3 ryanodine receptor.
PA1-026A was used in western blot to study the effect of FKBP12 on the activity of the type-3 ryanodine receptor.
|Van Acker K,Bultynck G,Rossi D,Sorrentino V,Boens N,Missiaen L,De Smedt H,Parys JB,Callewaert G||Journal of cell science (117:1129)||2004|
Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle.
PA1-026A was used in western blot to investigate the importance of RyR2 complex formation in the development of a metabolic crisis in malignant hyperthermia
|Glover L,Heffron JJ,Ohlendieck K||Journal of applied physiology (Bethesda, Md. : 1985) (96:11)||2004|
Transport activity and surface expression of the Na+-Ca2+ exchanger NCX1 are inhibited by the immunosuppressive agent cyclosporin A and by the nonimmunosuppressive agent PSC833.
PA1-026A was used in western blot to study the effects of cyclosporin A and PSC833 on the sodium/calcium exchanger NCX1 expression and activity
|Kimchi-Sarfaty C,Kasir J,Ambudkar SV,Rahamimoff H||The Journal of biological chemistry (277:2505)||2002|
Effects of the immunosuppressant FK506 on intracellular Ca2+ release and Ca2+ accumulation mechanisms.
PA1-026A was used in western blot to study the effects of immunosuppressant FK506 on calcium homeostasis.
|Bultynck G,De Smet P,Weidema AF,Ver Heyen M,Maes K,Callewaert G,Missiaen L,Parys JB,De Smedt H||The Journal of physiology (525 Pt 3:681)||2000|
Identification of a physical interaction between calcineurin and nuclear factor of activated T cells (NFATp).
PA1-026A was used in western blot to investigate the interaction between calcineurin and NFATp
|Wesselborg S,Fruman DA,Sagoo JK,Bierer BE,Burakoff SJ||The Journal of biological chemistry (271:1274)||1996|
Bone morphogenetic protein 7 regulates reactive gliosis in retinal astrocytes and Müller glia.
PA1-026A was used in immunohistochemistry - frozen section to test if bone morphogenetic proteins trigger reactive gliosis in retinal astrocytes and Muller glial cells
|Dharmarajan S,Gurel Z,Wang S,Sorenson CM,Sheibani N,Belecky-Adams TL||Molecular vision (20:1085)||2014|
Fkbp1a controls ventricular myocardium trabeculation and compaction by regulating endocardial Notch1 activity.
PA1-026A was used in immunohistochemistry to study the role of Fbbp1a-mediated regulation of Notch1 activity in ventricular myocardial trabeculation and compaction
|Chen H,Zhang W,Sun X,Yoshimoto M,Chen Z,Zhu W,Liu J,Shen Y,Yong W,Li D,Zhang J,Lin Y,Li B,VanDusen NJ,Snider P,Schwartz RJ,Conway SJ,Field LJ,Yoder MC,Firulli AB,Carlesso N,Towbin JA,Shou W||Development (Cambridge, England) (140:1946)||2013|
Targeting colorectal cancer stem cells with inducible caspase-9.
PA1-026A was used in immunohistochemistry to investigate the influence of dimerization of inducible caspase-9 in colorectal cancer stem cells
|Kemper K,Rodermond H,Colak S,Grandela C,Medema JP||Apoptosis : an international journal on programmed cell death (17:528)||2012|
Protection against ischemic brain damage in rats by immunophilin ligand GPI-1046.
PA1-026A was used in immunohistochemistry to evaluate the protective effect of immunophilin ligand GPI-1046 against ischemic brain damage in rats
|Li F,Omori N,Hayashi T,Jin G,Sato K,Nagano I,Shoji M,Abe K||Journal of neuroscience research (76:383)||2004|
Early decrease of the immunophilin FKBP 52 in the spinal cord of a transgenic model for amyotrophic lateral sclerosis.
PA1-026A was used in immunohistochemistry to investigate the effect of the downregulation of FKBP-52 on the pathogenesis of amyotrophic lateral sclerosis
|Manabe Y,Warita H,Murakami T,Shiote M,Hayashi T,Omori N,Nagano I,Shoji M,Abe K||Brain research (935:124)||2002|
Differential protein profiling as a potential multi-marker approach for TSE diagnosis.
PA1-026A was used in immunocytochemistry to study differential protein profiling as a potential multi-marker approach for transmissible spongiform encephalopathy diagnosis
|Barr JB,Watson M,Head MW,Ironside JW,Harris N,Hogarth C,Fraser JR,Barron R||BMC infectious diseases (9:null)||2009|