PA1-026A detects FK506 binding protein 12 kDa (FKBP12) in human and mouse tissues.
PA1-026A has been successfully used in Western blot, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 12 kDa protein representing FKBP12 from rat heart homogenate.
The PA1-026A immunogen is a synthetic peptide corresponding to the N-terminal residues G(1) V Q V E T I S P G D G R(13) of human FKBP12. PA1-026A immunizing peptide (Cat. # PEP-012) is available for use in neutralization and control experiments.
Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins.
FKBP12:FK506 complexes inhibit calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase which blocks T-cell activation by preventing lymphokine gene transcription. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. In interactions with members of the TGF beta family type I receptors, FKBP12 has been shown to exert an inhibitory effect on the signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 12 kDa FK506-binding protein; 12 kDa FKBP; calstabin 1; Calstabin-1; EC 18.104.22.168; FK506 Binding Protein 12; FK506 binding protein 1A, 12kDa; FK506 binding protein12; FK506-binding protein 1; FK506-binding protein 12; FK506-binding protein 1A; FK506-binding protein 1A (12kD); FK506-binding protein, T-cell, 12-kD; FKBP-1A; FKBP12-Exip3; Immunophi; Immunophilin FKBP12; Peptidyl-prolyl cis-trans isomerase FKBP1A; PPIase FKBP1A; protein kinase C inhibitor 2; Rotamase
Gene Aliases: Fkbp; FKBP-12; FKBP-1A; FKBP1; FKBP12; FKBP1A; PKC12; PKCI2; PPIASE