|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues G(206) R S K E Q R L K E Q L G A Q Q E(222) of human I-FLICE.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||3-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: Hela whole cell extract, HeLa cell lysate.
I-FLICE (inhibitor of FLICE) is a catalytically inactive form FLICE (Caspase 8) and Mch4/FLICE2 (Caspase 10) (1). I-FLICE shares sequence homologies to FLICE and Mch4/FLICE2 and binds to these two molecules as demonstrated by co-immunoprecipitation anlalysis. However, unlike the effector proteases FLICE and Mch4/FLICE2, I-FLICE does not bind to the adaptor molecule FADD. I-FLICE inhibits both TNFR-1 and CD-95 induced apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.