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Invitrogen
FLIP Polyclonal Antibody
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Product Details
PA5-19899
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A peptide corresponding to amino acids 180 to 230 at C-terminus of human FLIP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blot applications, this antibody detects a band at ~25kDa and ~35kDa. A suggested positive control is Jurkat cell lysate.
PA5-19899 can be used with blocking peptide PEP-0025.
Target Information
FLIP (CFLAR) is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells. It acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: c-FLIP; CASH; CASP8 and FADD-like apoptosis regulator; Caspase homolog; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; Casper; Cellular FLICE-like inhibitory protein; CLARP; DED-caspase homologue; FADD-like antiapoptotic molecule 1; FLAME-1; FLIP-L; apoptosis inhibitor; FLIP-S; apoptosis inhibitor; I-FLICE; Inhibitor of FLICE; MACH-related inducer of toxicity; MRIT; testis secretory sperm-binding protein Li 233m; unnamed protein product; Usurpin; usurpin beta
Gene Aliases: 2310024N18Rik; A430105C05Rik; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8AP1; Casper; CFLAR; cFLIP; CLARP; ENSMUSG00000072980; FLAME; FLAME-1; FLAME1; FLIP; Gm9845; I-FLICE; MRIT
UniProt ID: (Human) O15519, (Mouse) O35732
Entrez Gene ID: (Human) 8837, (Mouse) 12633
protease binding
cysteine-type endopeptidase activity
death receptor binding
protein binding
enzyme activator activity
cysteine-type peptidase activity
macromolecular complex binding
peptidase activator activity
protein complex binding
death effector domain binding
protein heterodimerization activity
cysteine-type endopeptidase activity involved in apoptotic signaling pathway
protease
response to hypoxia
proteolysis
apoptotic process
skeletal muscle tissue development
extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of cardiac muscle cell apoptotic process
positive regulation of neuron projection development
skeletal muscle atrophy
regulation of skeletal muscle satellite cell proliferation
skeletal myofibril assembly
macrophage differentiation
cellular response to insulin stimulus
response to testosterone
regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
skeletal muscle tissue regeneration
positive regulation of neuron apoptotic process
regulation of necroptotic process
positive regulation of ERK1 and ERK2 cascade
cellular response to epidermal growth factor stimulus
cellular response to estradiol stimulus
cellular response to hypoxia
cellular response to dexamethasone stimulus
cellular response to nitric oxide
positive regulation of glomerular mesangial cell proliferation
negative regulation of myoblast fusion
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of extracellular matrix organization
negative regulation of reactive oxygen species biosynthetic process
negative regulation of cellular response to transforming growth factor beta stimulus
negative regulation of hepatocyte apoptotic process
negative regulation of epithelial cell apoptotic process
positive regulation of hepatocyte proliferation
negative regulation of extrinsic apoptotic signaling pathway
activation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of peptidase activity
positive regulation of catalytic activity
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of necroptotic process
apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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