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          • Primary Antibodies ›
          • FMO2 Antibodies

          Invitrogen

          FMO2 Polyclonal Antibody

          View all (5) FMO2 antibodies

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          Cite FMO2 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          FMO2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          FMO2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          FMO2 Antibody (PA5-95318) in WB

          Western blot analysis of FMO2 using FMO2 Polyclonal Antibody (Product # PA5-95318). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel)/120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: rat lung tissue lysates. Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubat... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          FMO2 Antibody in Western Blot (WB)
          FMO2 Polyclonal Antibody

          Product Details

          PA5-95318

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide corresponding to a sequence at the N-terminus of human FMO2 (78-115aa FPNFLHNSKLLEYFRIFAKKFDLLKYIQFQTTVLSVRK).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2807121

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: rat lung tissue, mouse lung tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          The flavin-containing monooxygenases are NADPH-dependent enzymes that catalyze the oxidation of many drugs and xenobiotics. In most mammals, there is a flavin-containing monooxygenase that catalyzes the N-oxidation of some primary alkylamines through an N-hydroxylamine intermediate. However, in humans, this enzyme is truncated and is probably rapidly degraded. The protein encoded by this gene represents the truncated form and apparently has no catalytic activity. A functional allele found in African Americans has been reported, but no sequence evidence has been deposited to support the finding. This gene is found in a cluster with the FMO1, FMO3, and FMO4 genes on chromosome 1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Dimethylaniline monooxygenase [N-oxide-forming] 2; Dimethylaniline oxidase 2; flavin containing monooxygenase 2 (non-functional); Flavin-containing monooxygenase 2; FMO 1B1; FMO 2; FMO, pulmonary; Pulmonary flavin-containing monooxygenase 2; unnamed protein product

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          Gene Aliases: 2310008D08Rik; 2310042I22Rik; AW107733; FMO1B1; FMO2

          View more View less

          UniProt ID: (Human) Q99518, (Mouse) Q8K2I3, (Rat) Q6IRI9

          View more View less

          Entrez Gene ID: (Human) 2327, (Mouse) 55990, (Rat) 246245

          View more View less

          Function(s)
          monooxygenase activity N,N-dimethylaniline monooxygenase activity oxidoreductase activity flavin adenine dinucleotide binding NADP binding oxidoreductase oxygenase metabolite interconversion enzyme
          Process(es)
          organic acid metabolic process NADP metabolic process xenobiotic metabolic process toxin metabolic process negative regulation of fatty acid oxidation oxygen metabolic process energy homeostasis drug metabolic process oxidation-reduction process NADPH oxidation
          It has to be done as per old AB suggested Products section.

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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