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Invitrogen
FMRP Polyclonal Antibody
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FIGURE: 1 / 7
FMRP Antibody (PA5-34584) in ICC/IF
Immunofluorescence analysis of FMRP was performed using Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with FMRP Rabbit Polyclonal Antibody (Product # PA5-34584) at 5 µg/mL dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:200... View More
Product Details
PA5-34584
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Chicken,
Gerbil,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human FMRP.
Conjugate
Form
Liquid
Concentration
0.9 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
For Western Blot, this antibody has non-specific bands at 13 kDa,32 kDa and 68 kDa.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with orangutan based on sequence homology.
Target Information
FMR1 binds RNA and is associated with polysomes. The protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure. Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FMRP; fragile X mental retardation protein 1; fragile X mental retardation protein 1 homolog; fragile X mental retardation syndrome 1 homolog; fragile X mental retardation-1 protein; Fragile X messenger ribonucleoprotein; Fragile X messenger ribonucleoprotein 1; MGC87458; Protein FMR-1; ragile X mental retardation protein
Gene Aliases: Fmr-1; FMR1; FMRP; FRAXA; POF; POF1
UniProt ID: (Human) Q06787, (Rat) Q80WE1
Entrez Gene ID: (Human) 2332, (Rat) 24948, (Mouse) 14265
G-quadruplex RNA binding
chromatin binding
RNA binding
mRNA binding
mRNA 3'-UTR binding
protein binding
microtubule binding
poly(U) RNA binding
translation repressor activity
translation initiation factor binding
RNA strand annealing activity
poly(G) binding
methylated histone binding
siRNA binding
miRNA binding
RNA stem-loop binding
identical protein binding
protein homodimerization activity
ribosome binding
ion channel binding
poly(A) RNA binding
dynein binding
protein heterodimerization activity
mRNA 5'-UTR binding
sequence-specific mRNA binding
nucleic acid binding
translation factor
regulation of alternative mRNA splicing, via spliceosome
positive regulation of receptor internalization
mRNA processing
cellular response to DNA damage stimulus
glutamate receptor signaling pathway
RNA splicing
negative regulation of translation
gene silencing by RNA
positive regulation of histone phosphorylation
cellular response to UV
regulation of mRNA stability
modulation by host of viral RNA genome replication
positive regulation of translation
negative regulation of translational initiation
regulation of neurotransmitter secretion
mRNA transport
regulation of filopodium assembly
positive regulation of filopodium assembly
regulation of gene silencing by miRNA
regulation of dendritic spine development
positive regulation of dendritic spine development
cellular response to hydroxyurea
cellular response to virus
regulation of neuronal action potential
negative regulation of long term synaptic depression
positive regulation of intracellular transport of viral material
negative regulation of voltage-gated calcium channel activity
positive regulation of proteasomal protein catabolic process
positive regulation of mRNA binding
negative regulation of synaptic vesicle exocytosis
positive regulation of gene silencing by miRNA
negative regulation of cytoplasmic translation
positive regulation of response to DNA damage stimulus
regulation of translation
central nervous system development
transport
dendritic spine development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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