Recommended positive controls: 293T, A431, Jurkat, Raji.
Predicted reactivity: Mouse (97%), Rat (98%), Pig (97%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Eukaryotic cells contain 3 different types of prenyltransferases that attach either a farnesyl group (15 carbons) or a geranylgeranyl group (20 carbons) in thioether linkage to C-terminal cysteine residues in a variety of proteins. These posttranslational modifications provide a mechanism for membrane localization of proteins that lack a transmembrane domain. CAAX farnesyltransferase (FTase) attaches a farnesyl group from farnesyl pyrophosphate to cysteine residues at the fourth position from the C terminus of proteins that end in the CAAX box, where C is cysteine, A is usually but not always an aliphatic amino acid, and X is typically methionine or serine. This enzyme has the ability to farnesylate peptides as short as 4 residues in length that conform to the CAAX consensus sequence. The gene for the beta subunit of CAAX farnesyltransferase (FNTB) has been pinpointed to 14q23-q24 by Southern blot hybridization and PCR analyses of panels of human/Chinese hamster somatic cell hybrid lines and by fluorescence chromosomal in situ hybridization.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CAAX farnesyltransferase subunit beta; EC 188.8.131.52; ft beta; FTase beta; FTase-beta; Protein farnesyltransferase subunit beta; Ras proteins prenyltransferase subunit beta
Gene Aliases: FNTB; FPTB
UniProt ID: (Human) P49356
Entrez Gene ID: (Human) 2342
Molecular Function: acyltransferase