Invitrogen

FOXA1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

8 Published Figures

15 References

View all (43) FOXA1 antibodies

Datasheet

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Datasheet

Protocols

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FIGURE: 1 / 22

FOXA1 Antibody (PA5-27157) in WB

Western blot was performed using Anti-FOXA1 Polyclonal Antibody(Product # PA5-27157) and a 51kDa band corresponding to FOXA1 was observed across cell lines tested except SW480, HCT 116 and SH-SY5Y which is reported to be low. Whole Cell Extract (1% SDS) (30 µg lysate) of MCF7 (Lane 1), T-47D (Lane 2), HT-29 (Lane 3), SW480 (Lane 4), HCT 116 (Lane 5) and SH-SY5Y (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB230... View More

Product Image Gallery

FOXA1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of FOXA1 was performed using 70% confluent log phase LNCaP and SW480 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FOXA1 Polyclonal Antibody (Product # PA5-27157) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:3000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained withRhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nuclear localization. Panel e represents image for SW480 cells showing no staining for FOXA1. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

FOXA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — FOXA1 Polyclonal Antibody detects FOXA1 protein at nucleus on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. FOXA1 Polyclonal Antibody (Product # PA5-27157) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

FOXA1 Antibody in Immunoprecipitation (IP) — FOXA1 Polyclonal Antibody immunoprecipitates FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 µg whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of FOXA1 by 2.5 µg of FOXA1 Polyclonal Antibody (Product # PA5-27157) 10% SDS-PAGE The immunoprecipitated FOXA1 protein was detected by FOXA1 Polyclonal Antibody (Product # PA5-27157) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.

FOXA1 Antibody in ChIP Assay (ChIP) — Chromatin Immunoprecipitation (ChIP) assay of endogenous FOXA1 protein using FOXA1 Antibody: ChIP was performed using Anti-FOXA1 Polyclonal Antibody (Product # PA5-27157, 2.5 µg) on sheared chromatin from LnCaP cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to KLK3, SLµg, TMPRSS2 (Active) and SAT2 satellite repeats (Inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

FOXA1 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Extended Data Figure 4 Functional impact of FOXA1 mutations on oncogenic AR-signaling. a) Immunoblot showing expression of endogenous and V5-tagged exogenous FOXA1 proteins in dox-inducible 22RV1 cells transfected with distinct UTR-specific FOXA1 siRNAs (#3-5) or a non-targeting control siRNA (siNC). These results represent 2 independent experiments. Incucyte growth curves of 22RV1 cells overexpressing empty vector (control), WT, or mutant FOXA1 variants upon treatment with UTR-specific FOXA1-targeting siRNAs (n = 5 biological replicates). Mean +- s.e.m are shown. b) Immunoblots confirming stable overexpression of the WT AR protein in HEK293 and PC3 cells. c, d) Co-immunoprecipitation assay of indicated recombinant FOXA1 variants using a V5-tag antibody in (c) HEK293-AR and (d) PC3-AR cells. eGFP is a negative control; FL is the full-length WT FOXA1. d168 and d358 are truncated FOXA1 variants with only the first 168aa (i.e. before the Forkhead domain) or 358aa of FOXA1 protein. H247Q and R261G are missense class1 mutant variants. e) Immunoblots confirming comparable expression of AR and recombinant FOXA1 variants in AR reporter assay-matched HEK293 lysates. Immunoblots show representative results from 2-3 independent experiments and class1 and class2 mutants serve as biological replicates. For all gel source data ( a,b-e ), see Supplementary Figure 1 . f) AR dual-luciferase reporter assays with transient overexpression of indicated FOXA1 variant in HEK293-AR cells with or wit

FOXA1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of FOXA1 was observed in MCF-7, T-47D and HT-29 in comparison to SW480, HCT 116 and SH-SY5Y using Anti-FOXA1 Polyclonal Antibody (Product # PA5-27157) in Western Blot. {RE}

Product Details

PA5-27157

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:100-1:500

View 1 publication 1 publication

ChIP assay (ChIP)

Assay-dependent

View 3 publications 3 publications

Dot blot (DB)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 412 and 472 of FOXA1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544633

Product Specific Information

Recommended positive controls: A431, HepG2.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting SWI/SNF ATPases in enhancer-addicted prostate cancer.

Nature

Xiao L,Parolia A,Qiao Y,Bawa P,Eyunni S,Mannan R,Carson SE,Chang Y,Wang X,Zhang Y,Vo JN,Kregel S,Simko SA,Delekta AD,Jaber M,Zheng H,Apel IJ,McMurry L,Su F,Wang R,Zelenka-Wang S,Sasmal S,Khare L,Mukherjee S,Abbineni C,Aithal K,Bhakta MS,Ghurye J,Cao X,Navone NM,Nesvizhskii AI,Mehra R,Vaishampayan U,Blanchette M,Wang Y,Samajdar S,Ramachandra M,Chinnaiyan AM

PA5-27157 was used in Immunohistochemistry to study the efficacy of proteolysis-targeting chimera degrader, AU-15330, in degradation of SWI/SNF ATPase subunits, SMARCA2 and SMARCA4.

Sat Jan 01 00:00:00 EST 2022

Targeting SWI/SNF ATPases in enhancer-addicted prostate cancer.

Nature

PA5-27157 was used in Immunohistochemistry to study the efficacy of proteolysis-targeting chimera degrader, AU-15330, in degradation of SWI/SNF ATPase subunits, SMARCA2 and SMARCA4.

Sat Jan 01 00:00:00 EST 2022

STK11/LKB1 Loss of Function Is Associated with Global DNA Hypomethylation and S-Adenosyl-Methionine Depletion in Human Lung Adenocarcinoma.

Cancer research

Koenig MJ,Agana BA,Kaufman JM,Sharpnack MF,Wang WZ,Weigel C,Navarro FCP,Amann JM,Cacciato N,Arasada RR,Gerstein MB,Wysocki VH,Oakes C,Carbone DP

PA5-27157 was used in Immunocytochemistry to discover a novel association between LKB1 loss and widespread DNA demethylation.

Sun Aug 15 00:00:00 EDT 2021

Comprehensive Gene Expression Analyses of Immunohistochemically Defined Subgroups of Muscle-Invasive Urinary Bladder Urothelial Carcinoma.

International journal of molecular sciences

Kim B,Jang I,Kim K,Jung M,Lee C,Park JH,Kim YA,Moon KC

PA5-27157 was used in Immunohistochemistry to indicate that the CK5/6 positive subgroup exhibited high gene expression signature related to aggressive behavior and exhibited worse clinical outcome.

Sun Jan 10 00:00:00 EST 2021

PD-L1 Expression in Muscle-Invasive Urinary Bladder Urothelial Carcinoma According to Basal/Squamous-Like Phenotype.

Frontiers in oncology

Kim B,Lee C,Kim YA,Moon KC

PA5-27157 was used in Immunohistochemistry to evaluate PD-L1 expression by the SP142 and SP263 assays and classified the cases "positive" or "negative" according to the manufacturer's recommendations.

Thu Dec 31 00:00:00 EST 2020

Non-Muscle-Invasive Bladder Carcinoma with Respect to Basal Versus Luminal Keratin Expression.

International journal of molecular sciences

Jung M,Jang I,Kim K,Moon KC

PA5-27157 was used in Immunohistochemistry (Paraffin) to identify genetic signatures of NMIBC related to basal (K5/6) and luminal (K20) keratin expression.

Mon Oct 19 00:00:00 EDT 2020

Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer.

Nature

Parolia A,Cieslik M,Chu SC,Xiao L,Ouchi T,Zhang Y,Wang X,Vats P,Cao X,Pitchiaya S,Su F,Wang R,Feng FY,Wu YM,Lonigro RJ,Robinson DR,Chinnaiyan AM

Published figure using FOXA1 polyclonal antibody (Product # PA5-27157) in Western Blot

Mon Jul 01 00:00:00 EDT 2019

Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer.

Nature

Parolia A,Cieslik M,Chu SC,Xiao L,Ouchi T,Zhang Y,Wang X,Vats P,Cao X,Pitchiaya S,Su F,Wang R,Feng FY,Wu YM,Lonigro RJ,Robinson DR,Chinnaiyan AM

Published figure using FOXA1 polyclonal antibody (Product # PA5-27157) in Western Blot

Mon Jul 01 00:00:00 EDT 2019

Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer.

Nature

Parolia A,Cieslik M,Chu SC,Xiao L,Ouchi T,Zhang Y,Wang X,Vats P,Cao X,Pitchiaya S,Su F,Wang R,Feng FY,Wu YM,Lonigro RJ,Robinson DR,Chinnaiyan AM

Published figure using FOXA1 polyclonal antibody (Product # PA5-27157) in Western Blot

Mon Jul 01 00:00:00 EDT 2019

Androgen Receptor-Activated Enhancers Simultaneously Regulate Oncogene TMPRSS2 and lncRNA PRCAT38 in Prostate Cancer.

Cells

Chen Z,Song X,Li Q,Xie L,Guo T,Su T,Tang C,Chang X,Liang B,Huang D

Published figure using FOXA1 polyclonal antibody (Product # PA5-27157) in ChIP assay

Fri Aug 09 00:00:00 EDT 2019

Androgen Receptor-Activated Enhancers Simultaneously Regulate Oncogene TMPRSS2 and lncRNA PRCAT38 in Prostate Cancer.

Cells

Chen Z,Song X,Li Q,Xie L,Guo T,Su T,Tang C,Chang X,Liang B,Huang D

Published figure using FOXA1 polyclonal antibody (Product # PA5-27157) in ChIP assay

Fri Aug 09 00:00:00 EDT 2019

Aberrant activation of CYR61 enhancers in colorectal cancer development.

Journal of experimental & clinical cancer research : CR

Xie L,Song X,Lin H,Chen Z,Li Q,Guo T,Xu T,Su T,Xu M,Chang X,Wang LK,Liang B,Huang D

Published figure using FOXA1 polyclonal antibody (Product # PA5-27157) in Western Blot

Wed May 22 00:00:00 EDT 2019

Transcriptional Analysis of Immunohistochemically Defined Subgroups of Non-Muscle-Invasive Papillary High-Grade Upper Tract Urothelial Carcinoma.

International journal of molecular sciences

Jung M,Lee JH,Kim B,Park JH,Moon KC

PA5-27157 was used in Immunohistochemistry to facilitate the application of easily accessible prognostic biomarkers in practice.

Tue Jan 29 00:00:00 EST 2019

Analysis of the androgen receptor-regulated lncRNA landscape identifies a role for ARLNC1 in prostate cancer progression.

Nature genetics

Zhang Y,Pitchiaya S,Cieślik M,Niknafs YS,Tien JC,Hosono Y,Iyer MK,Yazdani S,Subramaniam S,Shukla SK,Jiang X,Wang L,Liu TY,Uhl M,Gawronski AR,Qiao Y,Xiao L,Dhanasekaran SM,Juckette KM,Kunju LP,Cao X,Patel U,Batish M,Shukla GC,Paulsen MT,Ljungman M,Jiang H,Mehra R,Backofen R,Sahinalp CS,Freier SM,Watt AT,Guo S,Wei JT,Feng FY,Malik R,Chinnaiyan AM

PA5-27157 was used in Chromatin immunoprecipitation to support a role for ARLNC1 in maintaining a positive feedback loop that potentiates AR signaling during prostate cancer progression and identify ARLNC1 as a novel therapeutic target.

Fri Jun 01 00:00:00 EDT 2018

FOXA1 knock-out via CRISPR/Cas9 altered Casp-9, Bax, CCND1, CDK4, and fibronectin expressions in LNCaP cells.

Experimental biology and medicine (Maywood, N.J.)

Albayrak G,Konac E,Ugras Dikmen A,Bilen CY

Published figure using FOXA1 polyclonal antibody (Product # PA5-27157) in Western Blot

Wed Aug 01 00:00:00 EDT 2018

FOXA1 knock-out via CRISPR/Cas9 altered Casp-9, Bax, CCND1, CDK4, and fibronectin expressions in LNCaP cells.

Experimental biology and medicine (Maywood, N.J.)

Albayrak G,Konac E,Ugras Dikmen A,Bilen CY

Published figure using FOXA1 polyclonal antibody (Product # PA5-27157) in Western Blot

Wed Aug 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Ci-fkh; fkd7; fkh; Forkhead box protein A1; forkhead-7; FoxA4a; Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; HNF3-alpha; HNF3alpha; MGC33105; MGC52590; MGC75967; pintallavis; TCF-3A; Transcription factor 3A; xfkh1; xfkh2

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Gene Aliases: FOXA1; HNF3A; TCF3A

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UniProt ID: (Human) P55317

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Entrez Gene ID: (Human) 3169

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Molecular Function: winged helix/forkhead transcription factor

Function(s)

negative regulation of transcription from RNA polymerase II promoter chromatin remodeling transcription from RNA polymerase II promoter Notch signaling pathway negative regulation of epithelial to mesenchymal transition covalent chromatin modification dorsal/ventral neural tube patterning response to estradiol positive regulation of intracellular estrogen receptor signaling pathway hormone metabolic process glucose homeostasis positive regulation of neuron differentiation positive regulation of smoothened signaling pathway positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter anatomical structure formation involved in morphogenesis neuron fate specification positive regulation of sequence-specific DNA binding transcription factor activity epithelial tube branching involved in lung morphogenesis lung epithelial cell differentiation secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development epithelial-mesenchymal signaling involved in prostate gland development prostate gland epithelium morphogenesis prostate gland stromal morphogenesis epithelial cell maturation involved in prostate gland development alveolar secondary septum development dopaminergic neuron differentiation respiratory basal cell differentiation positive regulation of cell-cell adhesion mediated by cadherin

Process(es)

RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor binding protein domain specific binding sequence-specific DNA binding transcription regulatory region DNA binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FOXA1 Polyclonal Antibody

FOXA1 Antibody (PA5-27157) in WB

Product Details

PA5-27157

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Dot blot (DB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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