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Invitrogen
FOXA1 Polyclonal Antibody
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FIGURE: 1 / 3
FOXA1 Antibody (PA5-79281) in WB
Western blot was performed using Anti-FOXA1 Polyclonal Antibody (Product # PA5-79281) and a 49 kDa band corresponding to FOXA1 was observed across cell lines tested except SW480, HCT 116 and SH-SY5Y which are reported to be low. An unspecified band around 20 kDa has also been observed. Modified whole cell extracts (1% SDS) (30 µg lysate) of MCF-7 (Lane 1), T-47D (Lane 2), HT-29 (Lane 3), SW480 (Lane 4), HCT 116 (Lane 5) and SH-SY5Y (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved protei... View More
Product Details
PA5-79281
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human FOXA1 recombinant protein (Position: M1-T55).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human MCF-7 whole cell, human HepG2 whole cell, human RT4 whole cell. Flow: RT4 cell.
Target Information
This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ci-fkh; fkd7; fkh; Forkhead box protein A1; forkhead-7; FoxA4a; Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; HNF3-alpha; HNF3alpha; MGC33105; MGC52590; MGC75967; pintallavis; TCF-3A; tFoxA1; Transcription factor 3A; xfkh1; xfkh2
Gene Aliases: FOXA1; HNF3A; TCF3A
UniProt ID: (Human) P55317
Entrez Gene ID: (Human) 3169
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor binding
protein domain specific binding
sequence-specific DNA binding
transcription regulatory region DNA binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
chromatin remodeling
transcription from RNA polymerase II promoter
Notch signaling pathway
negative regulation of epithelial to mesenchymal transition
covalent chromatin modification
dorsal/ventral neural tube patterning
response to estradiol
positive regulation of intracellular estrogen receptor signaling pathway
hormone metabolic process
glucose homeostasis
positive regulation of neuron differentiation
positive regulation of smoothened signaling pathway
positive regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
anatomical structure formation involved in morphogenesis
neuron fate specification
positive regulation of sequence-specific DNA binding transcription factor activity
epithelial tube branching involved in lung morphogenesis
lung epithelial cell differentiation
secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development
epithelial-mesenchymal signaling involved in prostate gland development
prostate gland epithelium morphogenesis
prostate gland stromal morphogenesis
epithelial cell maturation involved in prostate gland development
alveolar secondary septum development
dopaminergic neuron differentiation
respiratory basal cell differentiation
positive regulation of cell-cell adhesion mediated by cadherin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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