Search
Invitrogen
FOXA3 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
FOXA3 Antibody (PA1-813) in WB
Western blot analysis of FOXA3 was performed by loading 25 µg of HepG2 (lane 1) mouse liver (lane 2) and rat liver (lane 3) lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a FOXA3 polyclonal antibody (Product # PA1-813) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotti... View More
Product Details
PA1-813
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues S(4) V K M E A H D L A E W S Y Y P E A G E(23) of mouse FOXA3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-813 detects FOXA3 from human samples.
PA1-813 has been successfully used in Western blot and ChIP applications. By Western blot, PA1-813 detects a ~37 kDa band representing FOXA3 from mouse liver.
The PA1-813 immunogen is a synthetic peptide corresponding to residues S(4) V K M E A H D L A E W S Y Y P E A G E(23) of mouse FOXA3. This immunizing peptide (PEP-299) is available for use in neutralization and control experiments.
Target Information
FOXA3 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. The crystal structure of a similar protein in rat has been resolved.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Fork head-related protein FKH H3; Forkhead box protein A3; hepatocyte nuclear factor 3 gamma (winged helix transcription factor); hepatocyte nuclear factor 3, gamma; Hepatocyte nuclear factor 3-gamma; HNF-3-gamma; HNF-3G; TCF-3G; Transcription factor 3G
Gene Aliases: FKHH3; FOXA3; Hnf-3g; HNF3G; Tcf-3g; TCF3G
UniProt ID: (Human) P55318, (Mouse) P35584, (Rat) P32183
Entrez Gene ID: (Human) 3171, (Mouse) 15377, (Rat) 25100
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein domain specific binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
transcription factor binding
transcription regulatory region DNA binding
winged helix/forkhead transcription factor
cellular glucose homeostasis
chromatin organization
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
spermatogenesis
cellular response to starvation
anatomical structure morphogenesis
cell differentiation
positive regulation of transcription from RNA polymerase II promoter
hematopoietic stem cell homeostasis
transcription, DNA-templated
multicellular organism development
negative regulation of cell proliferation
chromatin modification
positive regulation of hepatocyte differentiation
response to nutrient levels
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support