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Invitrogen
FOXC1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
FOXC1 Antibody (PA1-807) in ICC/IF
Immunofluorescence analysis of FOXC1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXC1 Rabbit Polyclonal Antibody (Product # PA1-807) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a... View More
Product Details
PA1-807
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues A(423) V D D P L P D Y S L P(434) of human FOXC1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2247078
Product Specific Information
PA1-807 detects FOXC1 from human and mouse samples.
PA1-807 has been successfully used in Western blot procedures. By Western blot, PA1-807 detects a ~50 kDa band representing FOXC1 from HepG2 cells and mouse kidney cells. This antibody also detects a nonspecific band at ~45 kDa from HepG2 cells, and at ~60 kDa from mouse kidney samples.
The PA1-807 immunogen is a synthetic peptide corresponding to residues A(423) V D D P L P D Y S L P(434) of human FOXC1.
PA1-807 can be used with blocking peptide PEP-298.
Target Information
FOXC1 is a protein belonging to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this protein is not known, however, it plays a role in the regulation of FGF19-FGFR4-MAPK pathway to promote both the development and maintenance of anterior segment structures within the eye. Mutations in this gene cause various glaucoma, iridogoniodysgenesis anomaly, Peters anomaly that includes central corneal leukoma, absence of the posterior corneal stroma and Descemetmembrane and Axenfeld-Rieger anomaly characterized by posterior corneal embryotoxon, iris adhesion to the Schwalbe line, hypertelorism, hypoplasia of the malar bones, congenital absence of some teeth and mental retardation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: congenital hydrocephalus; forkhead box C1 protein; Forkhead box protein C1; forkhead, drosophila, homolog-like 7; forkhead-related activator 3; Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; forkhead/winged helix-like transcription factor 7; FREAC-3; HGNC:3800; Mesoderm/mesenchyme forkhead 1; MF-1; myeloid factor-delta; Transcription factor FKH-1
Gene Aliases: ARA; ch; fkh-1; Fkh1; FKHL7; FOXC1; FREAC-3; FREAC3; frkhda; IGDA; IHG1; IRID1; Mf1; Mf4; RIEG3
UniProt ID: (Human) Q12948, (Mouse) Q61572
Entrez Gene ID: (Human) 2296, (Mouse) 17300
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
transcription factor binding
DNA binding, bending
sequence-specific DNA binding
transcription regulatory region DNA binding
winged helix/forkhead transcription factor
skeletal system development
ossification
ovarian follicle development
eye development
ureteric bud development
in utero embryonic development
somitogenesis
lymph vessel development
blood vessel remodeling
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
Notch signaling pathway
brain development
heart development
cell proliferation
germ cell migration
mesenchymal cell development
neural crest cell development
cell migration
negative regulation of angiogenesis
collagen fibril organization
glycosaminoglycan metabolic process
lacrimal gland development
embryonic heart tube development
maintenance of lens transparency
vascular endothelial growth factor signaling pathway
odontogenesis of dentin-containing tooth
camera-type eye development
positive regulation of transcription, DNA-templated
negative regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
regulation of organ growth
vascular endothelial growth factor receptor signaling pathway
paraxial mesoderm formation
artery morphogenesis
regulation of blood vessel size
ventricular cardiac muscle tissue morphogenesis
cardiac muscle cell proliferation
negative regulation of lymphangiogenesis
positive regulation of hematopoietic progenitor cell differentiation
positive regulation of hematopoietic stem cell differentiation
negative regulation of apoptotic process involved in outflow tract morphogenesis
blood vessel development
kidney development
heart morphogenesis
transcription, DNA-templated
positive regulation of gene expression
mesenchymal cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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