Immunofluorescent analysis of FOXO6 showing staining in the cytoplasm and nucleus of U-251 cells using a FOXO6 polyclonal antibody (Product # PA5-35117) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/ml, 1 h at 37°C). Nuclei were stained with DAPI (blue) (10 ug/ml, 10 min).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 154-181 amino acids from the central region of human FOXO6|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
FOXO6 is a transcriptional activator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.