|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:1000|
|Western Blot (WB)||1:1000-1:5000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal residues 400-431 of human FOXP3.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody does not detect mouse FOXP3 in Western blot but does detect mouse tissue for IHC.
Suggested positive control: antigen standard for FOXP3 (transient overexpression lysate).
FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AIID; DIETER; Forkhead box P3; Forkhead box protein P3; foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; PIDX; SCURFIN; scurfy; XPID
Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; scurfin; sf; XPID
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