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Invitrogen
FSTL1 Monoclonal Antibody (C16)
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FIGURE: 1 / 5
FSTL1 Antibody (MA5-50975) in IHC (P)
Immunohistochemistry (Paraffin) analysis of FSTL1 in human cardiac muscle tissue using FSTL1 Monoclonal Antibody (C16) (Product # MA5-50975) (10 µg/mL) and using PBS intead of the primary antibody as a control. The sample was then stained with HRP-Goat anti-Mouse IgG polyclonal secondary antibody (2 µg/mL). DAB staining.
Product Details
MA5-50975
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
C16
Immunogen
Recombinant Human FSTL1 protein, Glu21-Ile308 (Accession #Q12841)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.05% Proclin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Follistatin-like protein 1 (FSTL1) is a widely-expressed, extracellular glycoprotein that is homologously grouped into the osteonectin (BM-40/SPARC) family of secreted proteins based on its possession of both a follistatin-like and extracellular calcium-binding domain. Initially identified as a TGF-beta-inducible protein in a cloned mouse osteoblast cell line, FSTL1 has since been implicated in an array of cell-type-specific functions, such as the regulation of proliferation, differentiation, apoptosis and migration, as well as a number of biological processes, including embryonic development, inflammatory response, angiogenesis, tumorigenesis, and immune disease pathogenesis. Highly conserved across mammalian species and widely expressed in human tissues, FSTL1 can be upregulated through signaling mediators of the innate immune system,such as TLR4 agonists and the arthritogenic cytokine IL-1beta via NFkappaB pathways, to stimulate the expression and secretion of pro-inflammatory cytokines, including TNF-alpha, IL-1beta, IL-6 and IL-8. While cells of mesenchymal lineage are capable of FSTL1 production, FSTL1 expression is notably absent from cells of hematopoietic lineage under normal physiological conditions. Macrophages and monocytes are, however, capable of taking up FSTL1 at sites of inflammation where FSTL1 stimulation can cause the expression of caspase-1 and its resultant enzymatic cleavage of active IL-1beta from pro-IL-1beta. Whereas the overexpression of FSTL1 has been noted as a substantial contributor to the progression of immune diseases like rheumatoid arthritis (RA) and osteoarthritis (OA), diminished FSTL1 serum levels have been identified as playing a significant part in both ovarian and endometrial carcinogenesis, where it directly affects cell proliferation, migration and invasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ50214; FLJ52277; Follistatin-like protein 1; Follistatin-related protein 1; unnamed protein product
Gene Aliases: FRP; FSL1; FSTL1; MIR198; OCC-1; OCC1; tsc36
UniProt ID: (Human) Q12841
Entrez Gene ID: (Human) 11167, (Pig) 110256417
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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