Invitrogen

FTO Polyclonal Antibody

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FTO Antibody in Western Blot (WB) — Western blot analysis of FTO in human brain lysate. Sample was incubated in FTO polyclonal antibody (Product # PA1-46310).

FTO Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (WB, ICC/IF, IHC) — Published figure using FTO polyclonal antibody (Product # PA1-46310) in Western Blot, Immunocytochemistry and Immunohistochemistry

Product Details

PA1-46310

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	Assay-Dependent	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Chicken, Human, Mouse, Non-human primate
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to an C-terminal portion within residues 400-505 of the human protein. [Swiss-Prot# Q9C0B1] View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	tris citrate/tris phosphate, pH 7-8
Contains	0.1% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2247041

Product Specific Information

Suggested positive control: human brain lysate, human brain protein.

Target Information

FTO is a novel protein and a member of the non-heme dioxygenase (Fe(II)-and 2-oxoglutarate-dependent dioxygenases) superfamily. Amino acid conservation patterns support this hypothesis and indicates that both 2-oxoglutarate and iron should be important for FTO function. Though not much is yet known about FTO, it is thought that the protein may somehow modify the activity of genes involved in metabolism and fat storage, which in turn may influence a person's risk of obesity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fat mass and obesity-associated (FTO) protein regulates adult neurogenesis.

Human molecular genetics

Li L,Zang L,Zhang F,Chen J,Shen H,Shu L,Liang F,Feng C,Chen D,Tao H,Xu T,Li Z,Kang Y,Wu H,Tang L,Zhang P,Jin P,Shu Q,Li X

Published figure using FTO polyclonal antibody (Product # PA1-46310) in Immunofluorescence

Sat Jul 01 00:00:00 EDT 2017

Fat mass and obesity-associated (FTO) protein regulates adult neurogenesis.

Human molecular genetics

Li L,Zang L,Zhang F,Chen J,Shen H,Shu L,Liang F,Feng C,Chen D,Tao H,Xu T,Li Z,Kang Y,Wu H,Tang L,Zhang P,Jin P,Shu Q,Li X

Published figure using FTO polyclonal antibody (Product # PA1-46310) in Immunofluorescence

Sat Jul 01 00:00:00 EDT 2017

Fat mass and obesity-associated (FTO) protein regulates adult neurogenesis.

Human molecular genetics

Li L,Zang L,Zhang F,Chen J,Shen H,Shu L,Liang F,Feng C,Chen D,Tao H,Xu T,Li Z,Kang Y,Wu H,Tang L,Zhang P,Jin P,Shu Q,Li X

Published figure using FTO polyclonal antibody (Product # PA1-46310) in Immunofluorescence

Sat Jul 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: AlkB homolog 9; alpha-ketoglutarate dependent dioxygenase FTO; Alpha-ketoglutarate-dependent dioxygenase FTO; fat mass and obesity associated; Fat mass and obesity-associated protein; fatso; m6A(m)-demethylase FTO; MGC5149; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; Protein fatso; protein fto; tRNA N1-methyl adenine demethylase FTO; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO

Gene Aliases: ALKBH9; AW743446; BMIQ14; FTO; GDFD; KIAA1752; mKIAA1752

UniProt ID: (Human) Q7Z785, (Mouse) Q8BGW1

Entrez Gene ID: (Human) 79068, (Chicken) 415718, (Mouse) 26383

Molecular Function: oxygenase

Function(s)

temperature homeostasis DNA dealkylation involved in DNA repair regulation of lipid storage oxidative single-stranded DNA demethylation oxidative single-stranded RNA demethylation regulation of multicellular organism growth RNA repair regulation of respiratory system process adipose tissue development regulation of white fat cell proliferation oxidative demethylation DNA demethylation regulation of brown fat cell differentiation DNA repair cellular response to DNA damage stimulus oxidation-reduction process

Process(es)

FTO Polyclonal Antibody

Product Details

PA1-46310

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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