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Invitrogen
FTO Polyclonal Antibody
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FIGURE: 1 / 3
FTO Antibody (PA5-85698) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of FTO was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: FTO Polyclonal Antibody (Product # PA5-85698) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-85698
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human FTO. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
2.05 mg/mL
Amount
205 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (83%), Rat (83%), Pig (85%), Rabbit (88%).
Positive Control: U87-MG, SK-N-SH, IMR32, SK-N-AS, 293T, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
FTO is a novel protein and a member of the non-heme dioxygenase (Fe(II)-and 2-oxoglutarate-dependent dioxygenases) superfamily. Amino acid conservation patterns support this hypothesis and indicates that both 2-oxoglutarate and iron should be important for FTO function. Though not much is yet known about FTO, it is thought that the protein may somehow modify the activity of genes involved in metabolism and fat storage, which in turn may influence a person's risk of obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AlkB homolog 9; Alpha-ketoglutarate-dependent dioxygenase FTO; fat mass and obesity associated; Fat mass and obesity-associated protein; fatso; intragenic FTO exon 9 containing transcript; m6A(m)-demethylase FTO; MGC5149; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; protein fto; tRNA N1-methyl adenine demethylase FTO; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO; unnamed protein product
Gene Aliases: ALKBH9; BMIQ14; FTO; GDFD; IFEX9; KIAA1752; RGD1305121
UniProt ID: (Human) Q9C0B1, (Rat) Q2A121
Entrez Gene ID: (Human) 79068, (Rat) 291905
protein binding
ferrous iron binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
transferase activity
oxidative RNA demethylase activity
oxidative DNA demethylase activity
metal ion binding
dioxygenase activity
RNA N6-methyladenosine dioxygenase activity
tRNA demethylase activity
molecular_function
DNA-N1-methyladenine dioxygenase activity
oxygenase
temperature homeostasis
DNA dealkylation involved in DNA repair
regulation of lipid storage
snRNA processing
regulation of multicellular organism growth
RNA repair
regulation of respiratory system process
adipose tissue development
mRNA destabilization
regulation of white fat cell proliferation
regulation of brown fat cell differentiation
oxidative single-stranded DNA demethylation
oxidative single-stranded RNA demethylation
oxidative demethylation
DNA demethylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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