Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
FTO Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 5
FTO Antibody (PA5-89791) in WB
Western Blot was performed using Anti-FTO Polyclonal Antibody (Product # PA5-89791) and a 58 and 58.2 kDa band corresponding to Alpha-ketoglutarate-dependent dioxygenase FTO was observed across cell lines and tissues tested . Nuclear enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), U-87 MG (Lane 2), Hep G2 (Lane 3), PC-3 (Lane 4), HeLa (Lane 5), MCF7 (Lane 6), Mouse Skeletal Muscle (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose... View More
Product Details
PA5-89791
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
0.35 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: 293T; Cellular Location: Nucleus, Nucleus speckle
Target Information
FTO is a novel protein and a member of the non-heme dioxygenase (Fe(II)-and 2-oxoglutarate-dependent dioxygenases) superfamily. Amino acid conservation patterns support this hypothesis and indicates that both 2-oxoglutarate and iron should be important for FTO function. Though not much is yet known about FTO, it is thought that the protein may somehow modify the activity of genes involved in metabolism and fat storage, which in turn may influence a person's risk of obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: AlkB homolog 9; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; fatso; m6A(m)-demethylase FTO; MGC5149; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; Protein fatso; protein fto; tRNA N1-methyl adenine demethylase FTO; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO
Gene Aliases: ALKBH9; AW743446; BMIQ14; FTO; GDFD; KIAA1752; mKIAA1752; RGD1305121
UniProt ID: (Human) Q9C0B1, (Rat) Q2A121, (Mouse) Q8BGW1
Entrez Gene ID: (Human) 79068, (Rat) 291905, (Mouse) 26383
ferrous iron binding
oxidative RNA demethylase activity
oxidative DNA demethylase activity
DNA-N1-methyladenine dioxygenase activity
RNA N6-methyladenosine dioxygenase activity
molecular_function
sulfonate dioxygenase activity
oxidoreductase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
metal ion binding
dioxygenase activity
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
oxygenase
temperature homeostasis
DNA dealkylation involved in DNA repair
regulation of lipid storage
oxidative single-stranded DNA demethylation
oxidative single-stranded RNA demethylation
regulation of multicellular organism growth
RNA repair
regulation of respiratory system process
adipose tissue development
regulation of white fat cell proliferation
oxidative demethylation
DNA demethylation
regulation of brown fat cell differentiation
DNA repair
cellular response to DNA damage stimulus
oxidation-reduction process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support