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FIGURE: 1 / 1
FUK Antibody (PA5-120540) in WB
Western blot analysis of FUK in extracts of various cell lines. Samples were incubated with FUK Polyclonal antibody (Product # PA5-120540) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-120540
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 795-1084 of human FUK (NP_659496.2).
Conjugate
Form
Liquid
Concentration
2.3 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914112
Product Specific Information
Positive test controls include: U-87MG, Mouse brain, Mouse kidney.^M
^M
Immunogen sequence: LLKAAFICAG IVHVHSELQL SEQLLRTFGG GFELHTWSEL PHGSGLGTSS ILAGTALAAL QRAAGRVVGT EALIHAVLHL EQVLTTGGGW QDQVGGLMPG IKVGRSRAQL PLKVEVEEVT VPEGFVQKLN DHLLLVYTGK TRLARNLLQD VLRSWYARLP AVVQNAHSLV RQTEECAEGF RQGSLPLLGQ CLTSYWEQKK LMAPGCEPLT VRRMMDVLAP HVHGQSLAGA GGGGFLYLLT KEPQQKEALE AVLAKTEGLG NYSIHLVEVD TQGLSLKLLG TEASTCCPFP
Target Information
The protein encoded by this gene belongs to the GHMP (galacto-, homoserine, mevalonate and phosphomevalonate) kinase family and catalyzes the phosphorylation of L-fucose to form beta-L-fucose 1-phosphate. This enzyme catalyzes the first step in the utilization of free L-fucose in glycoprotein and glycolipid synthesis. L-fucose may be important in mediating a number of cell-cell interactions such as blood group antigen recognition, inflammation, and metastatis. While several transcript variants may exist for this gene, the full-length nature of only one has been described to date.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fucokinase; L-fucose kinase; mFLJ00241 protein; UNQ5838
Gene Aliases: 1110046B12Rik; AI303278; FCSK; FUK
UniProt ID: (Human) Q8N0W3, (Mouse) Q7TMC8
Entrez Gene ID: (Human) 197258, (Mouse) 234730
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