|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an N-terminal region of the human FUS protein (within residues 1-150).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.1% BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:250|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The target sequence has 95% sequence homology with mouse and 91% sequence homology with bovine.
The protein originally described as fused in Sarcoma is actually related to a number of different cellular processes and correlated to several diseases. FUS binds DNA as a single or double strand, and acts as a site for transcription factors to recognize. Since FUS is well-documented to be involved in liposarcoma, lipoma and leukaemia, it is believed that when its normal housekeeping functions related to transcription are disrupted, normal cells become cancerous. Interestingly, FUS is also associated with the fatal neuron disorder amyotrophic lateral sclerosis; interruption of its normal RNA-binding functions leads to incorrect cellular localization and leads to pathologies of the neuron. Thus, FUS regulation of cellular functions is a critical component of maintenance of healthy cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.