Invitrogen

FUS Polyclonal Antibody

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Antibody Testing Data (4)
Published Figures (4)

FUS Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

FUS Antibody (PA5-23696) in ICC/IF

Immunocytochemistry analysis of FUS in MDA-MB231 cells. Samples were incubated in FUS polyclonal antibody (Product # PA5-23696) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

FUS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FUS Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

PA5-23696

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:10-1:50

View 1 publication 1 publication

Flow Cytometry (Flow)

1:10-1:50

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 499-526 amino acids from the C-terminal region of human FUS

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2541196

Product Specific Information

This antibody is predicted to react with bovine based on sequence homology.

Target Information

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Muscle cells of sporadic amyotrophic lateral sclerosis patients secrete neurotoxic vesicles.

Journal of cachexia, sarcopenia and muscle

Le Gall L,Duddy WJ,Martinat C,Mariot V,Connolly O,Milla V,Anakor E,Ouandaogo ZG,Millecamps S,Lainé J,Vijayakumar UG,Knoblach S,Raoul C,Lucas O,Loeffler JP,Bede P,Behin A,Blasco H,Bruneteau G,Del Mar Amador M,Devos D,Henriques A,Hesters A,Lacomblez L,Laforet P,Langlet T,Leblanc P,Le Forestier N,Maisonobe T,Meininger V,Robelin L,Salachas F,Stojkovic T,Querin G,Dumonceaux J,Butler Browne G,González De Aguilar JL,Duguez S,Pradat PF

PA5-23696 was used in Western Blot, Immunocytochemistry to test a hypothesis that muscle vesicles may be involved in ALS pathology.

Fri Apr 01 00:00:00 EDT 2022

Muscle cells of sporadic amyotrophic lateral sclerosis patients secrete neurotoxic vesicles.

Journal of cachexia, sarcopenia and muscle

PA5-23696 was used in Western Blot, Immunocytochemistry to test a hypothesis that muscle vesicles may be involved in ALS pathology.

Fri Apr 01 00:00:00 EDT 2022

FUS-induced circular RNA ZNF609 promotes tumorigenesis and progression via sponging miR-142-3p in lung cancer.

Journal of cellular physiology

Liu S,Yang N,Jiang X,Wang J,Dong J,Gao Y

Published figure using FUS polyclonal antibody (Product # PA5-23696) in Western Blot

Fri Jan 01 00:00:00 EST 2021

Extensive splicing changes in an ALS/FTD transgenic mouse model overexpressing cytoplasmic fused in sarcoma.

Scientific reports

Ito D,Taguchi R,Deguchi M,Ogasawara H,Inoue E

PA5-23696 was used in Immunohistochemistry to identify aberrant splicing regulation mediated by mutant FUS during the early stages of ALS/FTD.

Tue Mar 17 00:00:00 EDT 2020

The Bromodomain Protein 4 Contributes to the Regulation of Alternative Splicing.

Cell reports

Uppal S,Gegonne A,Chen Q,Thompson PS,Cheng D,Mu J,Meerzaman D,Misra HS,Singer DS

PA5-23696 was used in Western Blotting to identify that alternative splicing is assisted by bromodomain protein 4 through its interaction with the splicing machinery during transcription elongation.

Tue Nov 19 00:00:00 EST 2019

The Bromodomain Protein 4 Contributes to the Regulation of Alternative Splicing.

Cell reports

Uppal S,Gegonne A,Chen Q,Thompson PS,Cheng D,Mu J,Meerzaman D,Misra HS,Singer DS

PA5-23696 was used in Western Blotting to identify that alternative splicing is assisted by bromodomain protein 4 through its interaction with the splicing machinery during transcription elongation.

Tue Nov 19 00:00:00 EST 2019

Dendritic Homeostasis Disruption in a Novel Frontotemporal Dementia Mouse Model Expressing Cytoplasmic Fused in Sarcoma.

EBioMedicine

Shiihashi G,Ito D,Arai I,Kobayashi Y,Hayashi K,Otsuka S,Nakajima K,Yuzaki M,Itohara S,Suzuki N

Published figure using FUS polyclonal antibody (Product # PA5-23696) in Western Blot

Sun Oct 01 00:00:00 EDT 2017

Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice.

Brain : a journal of neurology

Shiihashi G,Ito D,Yagi T,Nihei Y,Ebine T,Suzuki N

PA523696 was used in immunohistochemistry and western blot to test if neuronal loss is caused by toxic gain-of-function cytoplasmic aggregates or loss of nuclear RNA-binding protein function in patients with sporadic and familial amyotrophic lateral sclerosis

Thu Sep 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: 75 kDa DNA-pairing protein; fus-like protein; fused in sarcoma; fusion gene in myxoid liposarcoma; fusion, derived from t(12;16) malignant liposarcoma; heterogeneous nuclear ribonucleoprotein P2; hnRNP P2; Oncogene FUS; Oncogene TLS; pigpen protein; POMp75; Protein pigpen; RNA-binding protein FUS; translocated in liposarcoma; Translocated in liposarcoma protein

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Gene Aliases: ALS6; D430004D17Rik; D930039C12Rik; ETM4; FUS; FUS1; HNRNPP2; POMP75; TLS

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UniProt ID: (Human) P35637, (Mouse) P56959

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Entrez Gene ID: (Human) 2521, (Mouse) 233908

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Function(s)

nucleotide binding DNA binding transcription coactivator activity RNA binding protein binding zinc ion binding estrogen receptor binding myosin V binding ionotropic glutamate receptor binding identical protein binding poly(A) RNA binding retinoid X receptor binding thyroid hormone receptor binding nucleic acid binding metal ion binding RNA metabolism protein

Process(es)

mRNA splicing, via spliceosome cellular response to calcium ion regulation of nucleic acid-templated transcription

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FUS Polyclonal Antibody

FUS Antibody (PA5-23696) in ICC/IF

Product Details

PA5-23696

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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