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FUT8 Antibody (PA5-100534) in ICC/IF
Immunocytochemistry analysis of FUT8 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with FUT8 polyclonal antibody (Product # PA5-100534) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100534
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human FUT8(Accession Q9BYC5), corresponding to amino acid residues G275-V325.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850043
Product Specific Information
Antibody detects endogenous levels of total FUT8.
Target Information
Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha (1,6) fucosyltransferase; Alpha-(1,6)-fucosyltransferase; alpha-1,6-fucosyltransferase; Alpha1-6FucT; EC 2.4.1.68; Fucosyltransferase 8; fucosyltransferase 8 (alpha (1,6) fucosyltransferase); GDP-fucose--glycoprotein fucosyltransferase; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase; MGC26465
Gene Aliases: FUT8
UniProt ID: (Human) Q9P2U5, (Mouse) Q9WTS2, (Rat) Q6EV76
Entrez Gene ID: (Human) 2530, (Mouse) 53618, (Rat) 432392
in utero embryonic development
protein N-linked glycosylation
N-glycan processing
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
respiratory gaseous exchange
oligosaccharide biosynthetic process
regulation of gene expression
cell migration
protein N-linked glycosylation via asparagine
protein glycosylation in Golgi
N-glycan fucosylation
L-fucose catabolic process
receptor metabolic process
GDP-L-fucose metabolic process
regulation of cellular response to oxidative stress
fucosylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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