Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human lung stained using a FUZ polyclonal antibody (Product # PA5-23209) at 15 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (338 TSTHFPPEPGPPEKTEDE 355) of FUZ protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||15 µg/ml|
|Western Blot (WB)||2-6 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
FUZ is the human homolog of Drosophila fy protein and belongs to the fuzzy family. The fy gene of Drosophila encodes a novel four-pass transmembrane protein that plays two roles in the development of hairs on the Drosophila wing. The first is to specify the correct orientation of the hair by restricting its initiation to the distal vertex of the cell. The second role is to permit the development of just a single cell hair by maintaining the integrity of the F-actin and microtubule arrays that are required for hair development. In humans, the protein is involved in cytoskeleton function and cell polarity in epithelial cells. The human fuz gene is localized on chromosome 19q13.33.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.