Invitrogen

FXN Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

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Datasheet

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Datasheet

Questions & Answers

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FXN Antibody in Western Blot (WB) — Western Blot analysis of FXN in extracts from normal (control) and FXN / Frataxin knockout (KO) 293T cells using FXN Polyclonal Antibody (Product # PA5-95829) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

FXN Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Frataxin, mitochondrial was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FXN Polyclonal Antibody (Product # PA5-95829) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic(mitochondrial like pattern) localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7C1115LZR).

FXN Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Frataxin, mitochondrial siRNA and decrease in signal intensity was observed in Western Blot application using Anti-FXN Polyclonal Antibody (Product # PA5-95829). {KD}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500 - 1:2000

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 42-210 of human FXN / Frataxin

Conjugate

Unconjugated

Form

Liquid

Concentration

3.55 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2807631

Product Specific Information

Immunogen sequence: LRTDIDATCT PRRASSNQRG LNQIWNVKKQ SVYLMNLRKS GTLGHPGSLD ETTYERLAEE TLDSLAEFFE DLADKPYTFE DYDVSFGSGV LTVKLGGDLG TYVINKQTPN KQIWLSSPSS GPKRYDWTGK NWVYSHDGVS LHELLAAELT KALKTKLDLS SLAYSGKDA; Positive Samples: K-562, U-87MG, Jurkat, Raji, DU-145, Mouse kidney, Mouse liver, Mouse heart; Cellular Location: Cytoplasm, Mitochondrion

Target Information

FXN is a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: d-FXN; Frataxin intermediate form; Frataxin mature form; Frataxin(56-210); Frataxin(78-210); Frataxin(81-210); Frataxin, mitochondrial; Friedreich ataxia; Friedreich ataxia protein; Fxn; i-FXN; m56-FXN; m78-FXN; m81-FXN

Gene Aliases: CyaY; FA; FARR; FRDA; FXN; RGD1565754; X25

UniProt ID: (Human) Q16595, (Mouse) O35943

Entrez Gene ID: (Human) 2395, (Rat) 499335, (Mouse) 14297

Molecular Function: cation transporter kinase transferase transporter