Western blot detection of FXR from 50 µg of mouse liver extract. Lane 1 is incubated with PA1-334 (1:500), and lane 2 has the antibody plus the neutralizing peptide (PEP-273).

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Immunogen Image FXR Polyclonal Antibody

Product Details

PA1-334

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues R(224) K N V K Q H A D Q T V N E D(238) of mouse FXR.

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2155042

Product Specific Information

PA1-334 detects FXR in mouse samples.

PA1-334 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~55 kDa protein representing FXR in mouse liver samples. An unknown band at ~60 kDa is also observed in western blots.

The PA1-334 immunogen is a synthetic peptide corresponding to residues R(224) K N V K Q H A D Q T V N E D(238) of mouse FXR. The PA1-334 immunizing peptide (Cat. # PEP-273) is available for use in neutralization and control experiments.

Target Information

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR plays an essential role in bile acid homeostasis through the regulation of genes involved in bile acid synthesis, conjugation and enterohepatic circulation. FXR also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. In the liver FXR activates transcription of the corepressor NR0B2 thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in BA synthesis) implicating at least in part histone demethylase KDM1A resulting in epigenomic repression, and SLC10A1/NTCP (involved in hepatic uptake of conjugated BAs). FXR activates transcription of SLC27A5/BACS and BAAT (involved in BA conjugation), ABCB11/BSEP (involved in bile salt export) by directly recruiting histone methyltransferase CARM1, and ABCC2/MRP2 (involved in secretion of conjugated BAs) and ABCB4 (involved in secretion of phosphatidylcholine in the small intestine). (From Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bile acid receptor; farnesoid X activated receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; MGC163445; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14

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Gene Aliases: AI957360; Bar; Fxr; HRR1; Nr1h4; RIP14; Rxrip14

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UniProt ID: (Mouse) Q60641

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Entrez Gene ID: (Mouse) 20186

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription factor binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding steroid hormone receptor activity receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding thyroid hormone receptor activity zinc ion binding bile acid binding bile acid receptor activity peptide binding sequence-specific DNA binding metal ion binding retinoid X receptor binding chenodeoxycholic acid binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter nitrogen catabolite activation of transcription from RNA polymerase II promoter regulation of carbohydrate metabolic process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter Notch signaling pathway bile acid metabolic process intracellular receptor signaling pathway regulation of urea metabolic process intracellular bile acid receptor signaling pathway steroid hormone mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter negative regulation of bile acid biosynthetic process cellular response to organonitrogen compound cellular response to bile acid positive regulation of glutamate metabolic process positive regulation of ammonia assimilation cycle

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FXR Polyclonal Antibody

FXR Antibody (PA1-334) in WB

Product Details

PA1-334

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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