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Invitrogen

FXR Polyclonal Antibody

View all (16) FXR antibodies

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FXR Polyclonal Antibody

Product Details

PA5-18620

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5 µg/mL
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-

Flow Cytometry (Flow)

10 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (KSCREKTELTPDQQ) corresponding to the internal amino acids of NR1H4 (aa 251-265).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10983673

Product Specific Information

This antibody is predicted to react with canine based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

Target Information

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR plays an essential role in bile acid homeostasis through the regulation of genes involved in bile acid synthesis, conjugation and enterohepatic circulation. FXR also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. In the liver FXR activates transcription of the corepressor NR0B2 thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in BA synthesis) implicating at least in part histone demethylase KDM1A resulting in epigenomic repression, and SLC10A1/NTCP (involved in hepatic uptake of conjugated BAs). FXR activates transcription of SLC27A5/BACS and BAAT (involved in BA conjugation), ABCB11/BSEP (involved in bile salt export) by directly recruiting histone methyltransferase CARM1, and ABCC2/MRP2 (involved in secretion of conjugated BAs) and ABCB4 (involved in secretion of phosphatidylcholine in the small intestine). (From Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bile acid receptor; farnesoid X nuclear receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; MGC163445; nuclear receptor; Nuclear receptor subfamily 1 group H member 4; putative new variant; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14; unnamed protein product

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Gene Aliases: BAR; FXR; HRR-1; HRR1; NR1H4; PFIC5; RIP14

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UniProt ID: (Human) Q96RI1

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Entrez Gene ID: (Human) 9971

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding zinc ion binding ligand-dependent nuclear receptor binding bile acid binding lithocholic acid receptor activity sequence-specific DNA binding metal ion binding retinoid X receptor binding chenodeoxycholic acid binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter cellular glucose homeostasis immune system process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter inflammatory response cell-cell junction assembly Notch signaling pathway bile acid metabolic process negative regulation of macromolecule biosynthetic process negative regulation of tumor necrosis factor-mediated signaling pathway negative regulation of very-low-density lipoprotein particle remodeling regulation of low-density lipoprotein particle clearance bile acid and bile salt transport cell differentiation intracellular receptor signaling pathway negative regulation of interferon-gamma production negative regulation of interleukin-1 production negative regulation of interleukin-2 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of interleukin-17 production toll-like receptor 9 signaling pathway regulation of urea metabolic process cellular triglyceride homeostasis positive regulation of insulin secretion involved in cellular response to glucose stimulus intracellular bile acid receptor signaling pathway glucose homeostasis cholesterol homeostasis defense response to bacterium negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of insulin receptor signaling pathway negative regulation of inflammatory response fatty acid homeostasis regulation of insulin secretion involved in cellular response to glucose stimulus triglyceride homeostasis regulation of bile acid biosynthetic process cellular response to lipopolysaccharide cellular response to fatty acid negative regulation of monocyte chemotactic protein-1 production regulation of cholesterol metabolic process cellular response to bile acid positive regulation of adipose tissue development positive regulation of phosphatidic acid biosynthetic process positive regulation of glutamate metabolic process positive regulation of ammonia assimilation cycle
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