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Invitrogen

FXR Polyclonal Antibody

View all (17) FXR antibodies

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Product Details

PA5-30951

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human NR1H4. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Amount

43 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2548425

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, A549, H1299, HCT116, MCF-7.

Predicted reactivity: Mouse (92%), Rat (91%), Pig (92%), Rabbit (96%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR plays an essential role in bile acid homeostasis through the regulation of genes involved in bile acid synthesis, conjugation and enterohepatic circulation. FXR also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. In the liver FXR activates transcription of the corepressor NR0B2 thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in BA synthesis) implicating at least in part histone demethylase KDM1A resulting in epigenomic repression, and SLC10A1/NTCP (involved in hepatic uptake of conjugated BAs). FXR activates transcription of SLC27A5/BACS and BAAT (involved in BA conjugation), ABCB11/BSEP (involved in bile salt export) by directly recruiting histone methyltransferase CARM1, and ABCC2/MRP2 (involved in secretion of conjugated BAs) and ABCB4 (involved in secretion of phosphatidylcholine in the small intestine). (From Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bile acid receptor; farnesoid X activated receptor; farnesoid X nuclear receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; MGC163445; nuclear receptor; Nuclear receptor subfamily 1 group H member 4; putative new variant; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14; unnamed protein product

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Gene Aliases: AI957360; BAR; FXR; HRR-1; HRR1; NR1H4; PFIC5; RIP14; Rxrip14

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UniProt ID: (Human) Q96RI1, (Rat) Q62735, (Mouse) Q60641

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Entrez Gene ID: (Human) 9971, (Rat) 60351, (Mouse) 20186

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding zinc ion binding ligand-dependent nuclear receptor binding bile acid binding lithocholic acid receptor activity sequence-specific DNA binding metal ion binding retinoid X receptor binding chenodeoxycholic acid binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription factor binding double-stranded DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding steroid hormone receptor activity receptor activity thyroid hormone receptor activity bile acid receptor activity peptide binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter regulation of carbohydrate metabolic process regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter bile acid metabolic process response to glucose intracellular receptor signaling pathway response to lipopolysaccharide regulation of urea metabolic process intracellular bile acid receptor signaling pathway steroid hormone mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter digestive tract development negative regulation of bile acid biosynthetic process cellular response to organonitrogen compound cellular response to bile acid positive regulation of glutamate metabolic process positive regulation of ammonia assimilation cycle nitrogen catabolite activation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated Notch signaling pathway cellular glucose homeostasis immune system process inflammatory response cell-cell junction assembly negative regulation of macromolecule biosynthetic process negative regulation of tumor necrosis factor-mediated signaling pathway negative regulation of very-low-density lipoprotein particle remodeling regulation of low-density lipoprotein particle clearance bile acid and bile salt transport cell differentiation negative regulation of interferon-gamma production negative regulation of interleukin-1 production negative regulation of interleukin-2 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of interleukin-17 production toll-like receptor 9 signaling pathway cellular triglyceride homeostasis positive regulation of insulin secretion involved in cellular response to glucose stimulus glucose homeostasis cholesterol homeostasis defense response to bacterium negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response positive regulation of transcription, DNA-templated positive regulation of insulin receptor signaling pathway negative regulation of inflammatory response fatty acid homeostasis regulation of insulin secretion involved in cellular response to glucose stimulus triglyceride homeostasis regulation of bile acid biosynthetic process cellular response to lipopolysaccharide cellular response to fatty acid negative regulation of monocyte chemotactic protein-1 production regulation of cholesterol metabolic process positive regulation of adipose tissue development positive regulation of phosphatidic acid biosynthetic process
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