Proteintech

FXYD6 Polyclonal Antibody

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FXYD6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

FXYD6 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FXYD6 Antibody in Flow Cytometry (Flow) — 1X10^6 SH-SY5Y cells were stained with 0.2ug FXYD6 antibody (15805-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

15805-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FXYD6 Fusion Protein Ag8538 (23-95 aa encoded by BC018652)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: KEKEMDPFH YDYQTLRIGG LVFAVVLFSV GILLILSRRC KCSFNQKPRA PGDEEAQVEN LITANATEPQ KAEN (23-95 aa encoded by BC018652)

Target Information

This gene encodes a member of the FXYD family of transmembrane proteins. This particular protein encodes phosphohippolin, which likely affects the activity of Na,K-ATPase. Multiple alternatively spliced transcript variants encoding the same protein have been described. Related pseudogenes have been identified on chromosomes 10 and X. Read-through transcripts have been observed between this locus and the downstream sodium/potassium-transporting ATPase subunit gamma (FXYD2, GeneID 486) locus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

FXYD6 Regulates Chemosensitivity by Mediating the Expression of Na+/K+-ATPase α1 and Affecting Cell Autophagy and Apoptosis in Colorectal Cancer.

BioMed research international

Luo W,Liu Q,Chen X,Liu H,Quan B,Lu J,Zhang K,Wang X

Mon Oct 25 00:00:00 EDT 2021

Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation.

Journal of the American Society of Nephrology : JASN

Dumas SJ,Meta E,Borri M,Goveia J,Rohlenova K,Conchinha NV,Falkenberg K,Teuwen LA,de Rooij L,Kalucka J,Chen R,Khan S,Taverna F,Lu W,Parys M,De Legher C,Vinckier S,Karakach TK,Schoonjans L,Lin L,Bolund L,Dewerchin M,Eelen G,Rabelink TJ,Li X,Luo Y,Carmeliet P

Wed Jan 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: FXYD domain-containing ion transport regulator 6; Phosphohippolin; PLM-like protein; Vascular endothelial cell-specific protein 6; VESP6

Gene Aliases: 0610030I18Rik; FXYD6; Php; Plp; UNQ521/PRO1056

UniProt ID: (Human) Q9H0Q3, (Mouse) Q9D164, (Rat) Q91XV6

Entrez Gene ID: (Human) 53826, (Mouse) 59095, (Rat) 63847

Molecular Function: primary active transporter