Bioss

FZD3/Frizzled 3 Polyclonal Antibody

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FZD3/Frizzled 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated ( bs-13239R ) at 1:200 overnight at 4°C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy)and used at 1:200 dilution for 40 minutes at 37°CDAPI(5 µg/mL) was used to stain the cell nuclei.

FZD3/Frizzled 3 Antibody in Western Blot (WB) — Lane 1: mouse pancreas lysate probed with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C.

Product Details

BS-13239R

Applications	Tested Dilution	Publications
Western Blot (WB)	1:300	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:500	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-
ELISA (ELISA)	1:500-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide derived from human FZD3/Frizzled 3, amino acids 121-220. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.09% sodium azide
Storage conditions	-20°C

Target Information

This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: frizzled 3; frizzled 3, seven transmembrane spanning receptor; frizzled family receptor 3; frizzled homolog 3; Frizzled-3; Fz-3; hFz3; mFz3

Gene Aliases: AU020229; D930050A07Rik; Fz-3; Fz3; FZD3

UniProt ID: (Human) Q9NPG1, (Mouse) Q61086

Entrez Gene ID: (Human) 7976, (Mouse) 14365, (Rat) 266715

Molecular Function: transmembrane signal receptor