Invitrogen

FZD7 Polyclonal Antibody

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FZD7 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of FZD7 in immersion fixed frozen sections of embryonic mouse gastrointestinal tract (11 d.p.c.). Samples were incubated in FZD7 polyclonal antibody (Product # PA5-47232) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).

FZD7 Antibody in Western Blot (WB) — Western blot analysis of FZD7 in HepG2 human hepatocellular carcinoma cell line. Samples were incubated in FZD7 polyclonal antibody (Product # PA5-47232) using a dilution of 0.25 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Frizzled‚7 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions.

FZD7 Antibody in Immunocytochemistry (ICC/IF) — Figure 3 Interaction between Wnt3 and Fzd7 in neuroblastoma N2a cells. ( A ). IHC for GFP (green), Fzd7 (red), and Wnt3 (blue) in N2a cells after 3 days of each shRNA transfection containing GFP marker ( Ctrl or Fzd7 shRNA) and following 4 h of Wnt3 (100 ng/mL) treatment ( n = 3 separate transfections). Arrow: shRNA-transfected GFP cells (insert: higher magnification image); arrowhead: non-transfected cells; scale bar: 100 mum. ( B ). Fzd7 mRNA expression levels in total N2a cells determined by qRT-PCR ( n = 10 repeats) when IHC was performed in A . Data of Fzd7 shRNA were normalized to Gapdh and then to Ctrl shRNA (100%). ( C - E ). Quantification of Fzd7 ( C ), Wnt3 ( D ), Gapdh ( E )-positive cells among total GFP cells (251-356 counted), normalized to the Ctrl shRNA ( n = 6-12 repeats). Data represent the mean and SEM. Statistics: Student's t -test; *: p < 0.05; ***: p < 0.005; n.s. : not significant.

Product Details

PA5-47232

Applications	Tested Dilution	Publications
Western Blot (WB)	0.25 µg/mL	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	5-15 µg/mL	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7 Gln33-Leu185 View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2607398

Product Specific Information

In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse (rm) Frizzled-2 and less than 5% cross-reactivity with rmFrizzled-3, rmFrizzled-4, rhFrizzled-5, rmFrizzled-6, rmFrizzled-8, and rmFrizzled-9 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Activation of Frizzled-7 attenuates blood-brain barrier disruption through Dvl/β-catenin/WISP1 signaling pathway after intracerebral hemorrhage in mice.

Fluids and barriers of the CNS

He W,Lu Q,Sherchan P,Huang L,Hu X,Zhang JH,Dai H,Tang J

PA5-47232 was used in Immunohistochemistry to investigate the role of Frizzled-7 signaling in blood-brain barrier preservation after intracerebral hemorrhage in mice.