Invitrogen

FZR1 Polyclonal Antibody

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FZR1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of FZR1 in 4% paraformaldehyde-fixed HeLa cells using FZR1 polyclonal antibody (Product # PA5-85303) at a dilution of 1:500. Sample was then incubated with phalloidin secondary antibody at a dilution of 1:100.

FZR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FZR1 in paraffin-embedded mouse heart using FZR1 polyclonal antibody (Product # PA5-85303) at a dilution of 1:500.

FZR1 Antibody in Western Blot (WB) — Western blot was performed using Anti-FZR1 Polyclonal Antibody (Product # PA5-85303) and a 55kDa band corresponding to Fizzy-related protein homolog was observed. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), T98G (Lane 2), T-47D (Lane 3), HCT 116 (Lane 4), Jurkat (Lane 5) and tissue extract (30 µg lysate) of Mouse Testis (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

PA5-85303

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the center region of human FZR1.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.26 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol, 1% BSA
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2792446

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (90%), Pig (97%), Chicken (94%), Rhesus Monkey (100%), Bovine (97%).

Positive Control: A431, A431 nuclear extract.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

FZR is a 7WD domain family member implicated in cell cycle regulation of Drosophila and yeast. It has been shown that the over expression of fzr proteins increases natural killer cell-mediated cell death and as a result, suppresses tumor growth. The fzr protein is essential for cyclosome activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDC20 homolog 1; CDC20-like 1b; CDC20-like protein 1; Cdh1/Hct1 homolog; cell division cycle 20 related 1; Fizzy-related protein homolog; Fzr; hCDH1

Gene Aliases: AW108046; CDC20C; CDH1; FYR; FZR; FZR1; FZR2; HCDH; HCDH1; KIAA1242

UniProt ID: (Human) Q9UM11, (Mouse) Q9R1K5

Entrez Gene ID: (Human) 51343, (Mouse) 56371, (Rat) 314642

Molecular Function: ubiquitin-protein ligase