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          • Primary Antibodies ›
          • Factor IX Antibodies

          Invitrogen

          Factor IX Monoclonal Antibody (5041)

          1 Published Figure
          5 References
          View all (21) Factor IX antibodies

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          Cite Factor IX Monoclonal Antibody (5041)

          Additional Information:
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          • Published Figures (1)
          Factor IX Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.
          Factor IX Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Factor IX Antibody (MA1-43012) in ELISA

          Nature communications 2020 - Fig. 3 F9 KI into the alpha-globin locus results in expression and secretion of functional enzyme. a AAV6 donor used for KI experiments of FIX Padua. b FIX KI efficiency in HUDEP-2 cells was measured by flow cytometry (light green) or ddPCR specific for on-target integration (dark green) before and after sorting ( n = 1). c Quantification of FIX mRNA in KI HUDEP-2 upon differentiation (mean +- SD, n = 2 und... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Factor IX Antibody in ELISA (ELISA)

          Product Details

          MA1-43012

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          5 µg/mL
          -

          Immunohistochemistry (IHC)

          Assay-dependent
          View 1 publication 1 publication

          ELISA (ELISA)

          5-10 µg/mL
          View 4 publications 4 publications

          Radioimmune Assays (RIA)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human

          Published species

          Cynomolgus monkey, Human, Mouse

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          5041

          Immunogen

          Heavy chain of human factor IX and Ixa.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          9.6 mg/mL

          Amount

          100 µg

          Purification

          SDS-PAGE

          Storage buffer

          50% water with 50% glycerol

          Contains

          no preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1074312

          Product Specific Information

          MA1-43012 detects Factor IX in human samples, human factor Ixa and the heavy chain of human factor IX and IXa.

          MA1-43012 has successfully been used in radioimmune assays, ELISA, Western blot and immunohistochemistry applications.

          MA1-43012 was derived from human factor IX.

          Target Information

          Coagulation factor IX is a single chain polypeptide with a molecular mass of 57 kDa. Normal concentrations of factor IX in human plasma is approximately 3 µg/mL. Factor IX deficiency and lack of coagulation activity is seen in patients suffering from the innate X-chromosome linked disorder, haemophilia B.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Christmas factor; coagulation factor; Coagulation factor IX; coagulation factor IX precursor; EC 3.4.21.22; factor 9; factor IX (Christmas factor) precursor; factor IX F9; Factor9; FIX F9; plasma thromboplastic component; Plasma thromboplastin component; PTC; RP6-88D7.1; serine protease; unnamed protein product

          View more View less

          Gene Aliases: F9; F9 p22; FIX; HEMB; P19; PTC; THPH8

          View more View less

          UniProt ID: (Human) P00740

          View more View less

          Entrez Gene ID: (Human) 2158

          View more View less

          Function(s)
          endopeptidase activity serine-type endopeptidase activity calcium ion binding protein binding peptidase activity serine-type peptidase activity hydrolase activity metal ion binding
          Process(es)
          proteolysis blood coagulation hemostasis zymogen activation
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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