|Tested species reactivity||Rabbit, Dog, Human, Pig|
|Host / Isotype||Sheep / IgG|
|Immunogen||Factor V from human plasma.|
|Storage buffer||0.01M HEPES, pH 7.4, with 0.15M NaCl, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Inhibition Assays (IA)||Assay Dependent|
|Immunoelectrophoresis (IE)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-74106 detects Factor V from human samples.
The neutralizing activity of PA1-74106 is 27.5 Bethesda Units/ml IgG aginst normal plasma.
PA1-74106 has been successfully used in ELISA, immunoelectrophoresis, and inhibition assay applications.
The PA1-74106 immunogen is Factor V from human plasma.
Factor V (formerly referred to as accelerator globulin and labile factor) is a large glycoprotein (320kDa) that is produced in the liver. The gene that encodes factor V (F.V) is located on the chromosome 1. A congenital deficiency of F.V is a hemorrhagic disorder inherited as an autosomal recessive disease.
The concentration of F.V in plasma is typically 10µg/ml. F.V is a pro-cofactor that is activated through limited proteolysis by thrombin, or by activated factor X in the proscence of phospholipid surface. Other physiologic activators of F.V include plasmin, neutrophil elastase and platelet calpain. The activated cofactor (F.Va) is an essential component of the prothrombin activator complex, which consists of F.Va, activated factor X, calcium and anionic phospholipid surface. The intact prothrombinase complex activates prothrombin to thrombin at a rate 300,000-fold greater than activated factor X alone. In a positive feedback loop, the thrombin generated accelerates its own generation by activating more F.V to F.Va. Thrombin also acts to down regulate F.Va indirectly by activating protein C, which inactivates F.Va cofactor activity^1-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.