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Invitrogen
Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience™
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FIGURE: 1 / 1
Fc Receptor Binding Inhibitor Antibody (14-9162-42) in Flow
Normal canine peripheral blood cells were unstained (orange histogram), stained with Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (blue histogram), or were pretreated with Canine Fc Receptor Binding Inhibitor Purified and then stained with Rat IgG2b K Isotype Control FITC (purple histogram).Cells in the monocyte gate were used for analysis.
Product Details
14-9162-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog
Published species
Dog
Class
Polyclonal
Type
Antibody
Conjugate
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The Canine Fc Receptor Binding Inhibitor can be used to inhibit the non-specific Fc receptor (FcR)-mediated binding of rat monoclonal antibodies used for flow cytometric analysis of canine tissue. FcR are expressed at varying levels in multiple cell lineages including high expression in myeloid cells, granulocytes, and B cells. The biological function of the FcR, including initiation of endocytosis, phagocytosis, and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which rat monoclonal antibodies will bind to canine FcR varies depending on the isotype of the monoclonal antibody.
eBioscience testing indicates that rat IgG2b isotype is blocked by Canine Fc Receptor Binding Inhibitor.
Applications Reported: Canine Fc Receptor Binding Inhibitor Purified has been reported for use in flow cytometric analysis.
To inhibit the non-specific binding of rat monoclonal antibodies add the canine Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing, proceed to stain with primary antibody.
Applications Tested: Canine Fc Receptor Binding Inhibitor Purified has been pre-titrated and tested by flow cytometric analysis on canine peripheral blood cells. This can be used at 5 µL (10 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Fc blocking; FcR BI; FcR Inhibitor
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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