PA1-752 detects Fe65 from purified mouse samples. This antibody does not detect endogenous levels of Fe65.
PA1-752 has been successfully used in Western blot procedures. By Western blot, this antibody detects an 97 kDa protein representing Fe65 from immunopurified mouse brain lysate.
The PA1-752 immunizing peptide corresponds to amino acid residues 352-369 from mouse Fe65. This peptide (Cat. # PEP-200) is available for use in neutralization and control experiments.
The major transcript of the Fe65 gene is a neuron-specific mRNA that encodes a nuclear protein whose amino-terminal domain strongly activates the transcription of reporter genes. Fe65 has been implicated in a regulatory and cell signaling mechanism because it contains two different motifs involved in protein binding; a WW domain (a variant of Src homology 3 domains) and a phosphotyrosine interaction domain (PID). Interestingly, the PID domain binds to the same motif present in the conserved cytoplasmic domains of the beta PP and beta PP-like proteins.
RNA analyses revealed that Fe65 is predominantly expressed in the brain and in the regions most affected by Alzheimer's disease-associated neuropathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: adaptor protein FE65a2; amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65); amyloid beta A4 precursor protein-binding family B member 1; Amyloid-beta A4 precursor protein-binding family B member 1; APBB1; Protein Fe65; stat-like protein
Gene Aliases: APBB1; FE65; MGC:9072; RIR
Molecular Function: signaling molecule