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Invitrogen
Fetuin A Polyclonal Antibody
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FIGURE: 1 / 1
Fetuin A Antibody (PA5-95214) in WB
Western blot analysis of Fetuin A/AHSG in, Lane 1: mouse plasma lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with Fetuin A Polyclonal Antibody (Product # PA5-95214) at 0.5 μg/... View More
Product Details
PA5-95214
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived mouse Fetuin A recombinant protein (Position: A19-I345).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Mouse Fetuin A shares 57% and 85% amino acid (aa) sequence identity with human and rat Fetuin A, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse plasma.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-2-HS-glycoprotein; Countertrypin; Fetuin-A; FetuinA
Gene Aliases: Ahsg; Fetua
UniProt ID: (Mouse) P29699
Entrez Gene ID: (Mouse) 11625
ossification
protein complex assembly
acute-phase response
male gonad development
negative regulation of cell growth
negative regulation of bone mineralization
positive regulation of bone resorption
negative regulation of insulin receptor signaling pathway
regulation of inflammatory response
positive regulation of phagocytosis
negative regulation of protein tyrosine kinase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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