Invitrogen

Fetuin B Polyclonal Antibody

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Fetuin B Antibody — Figure 5. Alterations in expression of proteases and protease inhibitors in oviducts lacking epithelial estrogen receptor alpha (ERalpha). Real-time PCR of the indicated ( A ) proteases and ( B ) protease inhibitors in wild-type (WT) and conditional knockout (cKO) oviducts at 0.5 dpc (n = 4-7 mice/group; mean +- SEM). ( C ) Immunoblot analysis of fetuin B in WT and cKO oviducts; beta-actin served as a loading control. Protein lysate from one mouse in each lane; n = 4-5 mice/group. ( D ) Quantitation of fetuin B signal intensity normalized to beta-actin. ( E ) Fetuin B localization in WT and cKO oviducts at 0.5 dpc. Images shown are representative of n = 4 mice/group. Scale bar = 50 mum. For all panels, asterisk indicates significant difference compared to WT, p<0.05. dpc: Days post coitum. DOI: http://dx.doi.org/ Figure 5--figure supplement 1. Comparable expression of proteases and protease inhibitors in wild-type (WT) and mesenchymal conditional knockout (cKO) oviduct at 0.5 dpc. ( A ) Real-time PCR of the indicated proteases and protease inhibitors in WT and mesenchymal cKO oviducts at 0.5 dpc (n = 4-6 mice/group; mean +- SEM). ( B ) Immunoblot analysis and ( C ) normalized signal intensities of fetuin B protein to beta-actin in WT and mesenchymal cKO oviducts at 0.5 dpc. dpc: Days post coitum. DOI: http://dx.doi.org/

Fetuin B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Fetuin B in paraffin-embedded hepatoma using a Fetuin B polyclonal antibody (Product # PA5-29468) at a dilution of 1:100.

Fetuin B Antibody in Western Blot (WB) — Western blot analysis of Fetuin B using Various whole cell extracts (30 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with a Fetuin B polyclonal antibody (Product # PA5-29468) at a dilution of 1:1000.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Published Applications

Miscellaneous PubMed (MISC)

See 1 publication below

Immunohistochemistry (IHC)

See 1 publication below

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 86 and 361 of Human Fetuin B

Conjugate

Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2546944

Product Specific Information

PA5-29468 targets Fetuin B in IHC (P) and WB applications and shows reactivity with Human and mouse samples.

The PA5-29468 immunogen is recombinant fragment corresponding to a region within amino acids 86 and 361 of Human Fetuin B.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.