Invitrogen

Fetuin B Polyclonal Antibody

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Fetuin B Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of Fetuin B was performed in SK N_SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Fetuin B Polyclonal Antibody (Product # PA5 29468) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Fetuin B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Fetuin B in paraffin-embedded hepatoma using a Fetuin B polyclonal antibody (Product # PA5-29468) at a dilution of 1:100.

Fetuin B Antibody in Western Blot (WB) — Western blot analysis of Fetuin B was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Fetuin B Polyclonal Antibody (Product # PA5-29468) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Fetuin B Antibody in Immunoprecipitation (IP) — Immunoprecipitation of Fetuin B was performed in SK-N-SH whole cell extracts using 5 µg of Fetuin B Polyclonal Antibody (Product # PA5-29468). Samples were transferred to a membrane and probed with Fetuin B Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

Fetuin B Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 5. Alterations in expression of proteases and protease inhibitors in oviducts lacking epithelial estrogen receptor alpha (ERalpha). Real-time PCR of the indicated ( A ) proteases and ( B ) protease inhibitors in wild-type (WT) and conditional knockout (cKO) oviducts at 0.5 dpc (n = 4-7 mice/group; mean +- SEM). ( C ) Immunoblot analysis of fetuin B in WT and cKO oviducts; beta-actin served as a loading control. Protein lysate from one mouse in each lane; n = 4-5 mice/group. ( D ) Quantitation of fetuin B signal intensity normalized to beta-actin. ( E ) Fetuin B localization in WT and cKO oviducts at 0.5 dpc. Images shown are representative of n = 4 mice/group. Scale bar = 50 mum. For all panels, asterisk indicates significant difference compared to WT, p<0.05. dpc: Days post coitum. DOI: http://dx.doi.org/ Figure 5--figure supplement 1. Comparable expression of proteases and protease inhibitors in wild-type (WT) and mesenchymal conditional knockout (cKO) oviduct at 0.5 dpc. ( A ) Real-time PCR of the indicated proteases and protease inhibitors in WT and mesenchymal cKO oviducts at 0.5 dpc (n = 4-6 mice/group; mean +- SEM). ( B ) Immunoblot analysis and ( C ) normalized signal intensities of fetuin B protein to beta-actin in WT and mesenchymal cKO oviducts at 0.5 dpc. dpc: Days post coitum. DOI: http://dx.doi.org/

Product Details

PA5-29468

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:500	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 86 and 361 of Human Fetuin B View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.31 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% Proclin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2546944

Product Specific Information

Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse plasma, rat plasma.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Oviductal estrogen receptor α signaling prevents protease-mediated embryo death.

eLife

Winuthayanon W,Bernhardt ML,Padilla-Banks E,Myers PH,Edin ML,Lih FB,Hewitt SC,Korach KS,Williams CJ

PA5-29468 was used in immunohistochemistry to use transgenic mice to investigate how cyclic steroid hormone-mediated signals regulate fertilization and preimplantation development

Tue Dec 01 00:00:00 EST 2015

Oviductal estrogen receptor α signaling prevents protease-mediated embryo death.

eLife

Winuthayanon W,Bernhardt ML,Padilla-Banks E,Myers PH,Edin ML,Lih FB,Hewitt SC,Korach KS,Williams CJ

PA5-29468 was used in immunohistochemistry to use transgenic mice to investigate how cyclic steroid hormone-mediated signals regulate fertilization and preimplantation development

Tue Dec 01 00:00:00 EST 2015

Oviductal estrogen receptor α signaling prevents protease-mediated embryo death.

eLife

Winuthayanon W,Bernhardt ML,Padilla-Banks E,Myers PH,Edin ML,Lih FB,Hewitt SC,Korach KS,Williams CJ

PA5-29468 was used in immunohistochemistry to use transgenic mice to investigate how cyclic steroid hormone-mediated signals regulate fertilization and preimplantation development