|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Western Blot (WB)||1:1000-1:2000|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||C. elegans, Fruit fly, Human, Mouse, Plant, Rat, Yeast|
|Published species reactivity||Plant|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Yeast nuclear preparation (S cerevisiae)Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S cerevisiae)|
|Storage buffer||tissue culture supernatant|
|Contains||10mM sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-22000 detects fibrillarin in human, mouse, rat, plants, S. pombe, C. elegans and drosophila samples.
MA1-22000 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), and Western blot procedures.
The MA1-22000 immunogen is a yeast nuclear preparation (S cerevisiae). Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S cerevisiae).
Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; CG5725-PA; CG5725-PB; CG5725-PC; CG5725-PD; CG5725-PE; CG5725-PF; CG5725-PG; fbl-PA; fbl-PB; fbl-PC; fbl-PD; fbl-PE; fbl-PF; fbl-PG; FIB; FLRN; fumble; Histone-glutamine methyltransferase; Nucleolar protein 1; pantothenate kinase; RNA, U3 small nucleolar interacting protein 1; RNU3IP1; rRNA 2'-O-methyltransferase fibrillarin
Gene Aliases: AL022665; CELE_T01C3.7; CG5725; Dmel\CG5725; Dmel_CG5725; dPANK; dPANK/Fbl; FBL; FIB; fib-1; FIB1; FLRN; PANK; RNU3IP1; T01C3.7
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