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Immunofluorescent analysis of Fibrillarin in SH-SY5Y cells using a Fibrillarin monoclonal antibody (Product # MA1-22000) (green) at a dilution of 1:100,000 and with a chicken antibody to neurofilament NF-H (red) at a dilution of 1:1000. Nuclei were counterstained with Hoechst (blue).
|Tested species reactivity||C. elegans, Fruit fly, Human, Mouse, Plant, Rat, Yeast|
|Published species reactivity||Plant|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Yeast nuclear preparation (S cerevisiae)Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S cerevisiae)|
|Storage buffer||tissue culture supernatant|
|Contains||10mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Western Blot (WB)||1:1000-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA1-22000 detects fibrillarin in human, mouse, rat, plants, S. pombe, C. elegans and drosophila samples.
MA1-22000 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), and Western blot procedures.
The MA1-22000 immunogen is a yeast nuclear preparation (S cerevisiae). Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S cerevisiae).
Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
40S ribosome biogenesis co-factors are essential for gametophyte and embryo development.
MA1-22000 was used in immunohistochemistry to study the roles of 5 potential co-factors of Arabidopsis ribosome biogenesis
|Missbach S,Weis BL,Martin R,Simm S,Bohnsack MT,Schleiff E||PloS one (8:null)||2013|
34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; CG5725-PA; CG5725-PB; CG5725-PC; CG5725-PD; CG5725-PE; CG5725-PF; CG5725-PG; fbl-PA; fbl-PB; fbl-PC; fbl-PD; fbl-PE; fbl-PF; fbl-PG; FIB; FLRN; fumble; Histone-glutamine methyltransferase; Nucleolar protein 1; pantothenate kinase; RNA, U3 small nucleolar interacting protein 1; RNU3IP1; rRNA 2'-O-methyltransferase fibrillarin
AL022665; CELE_T01C3.7; CG5725; Dmel\CG5725; Dmel_CG5725; dPANK; dPANK/Fbl; FBL; FIB; fib-1; FIB1; FLRN; PANK; RNU3IP1; T01C3.7