MA1-22000 detects fibrillarin in human, mouse, rat, plants, S. pombe, C. elegans and drosophila samples.
MA1-22000 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), and Western blot procedures.
The MA1-22000 immunogen is a yeast nuclear preparation (S cerevisiae). Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S cerevisiae).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; CG5725-PA; CG5725-PB; CG5725-PC; CG5725-PD; CG5725-PE; CG5725-PF; CG5725-PG; fbl-PA; fbl-PB; fbl-PC; fbl-PD; fbl-PE; fbl-PF; fbl-PG; FIB; FLRN; fumble; Histone-glutamine methyltransferase; Nucleolar protein 1; pantothenate kinase; RNA, U3 small nucleolar interacting protein 1; RNU3IP1; rRNA 2'-O-methyltransferase fibrillarin
Gene Aliases: AL022665; CELE_T01C3.7; CG5725; Dmel\CG5725; Dmel_CG5725; dPANK; dPANK/Fbl; FBL; FIB; fib-1; FIB1; FLRN; PANK; RNU3IP1; T01C3.7