|Tested species reactivity||Human|
|Host / Isotype||Chicken / IgY|
|Immunogen||Mixture of synthetic peptides based on human fibrinogen.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:1000 - 1:5000|
|Western Blot (WB)||1:1000 - 1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-9526 detects Fibrinogen in human samples.
PA1-9526 has been successfully used in Western blot and ELISA procedures. By Western blot, PA1-9526 detects a ~94 kDa protein representing Fibrinogen.
The PA1-9526 immunogen is a mixture of synthetic peptides based on human fibrinogen.
Fibrinogen, the precursor of fibrin, is the coagulable protein in the blood plasma of vertebrates. Fibrinogen consists as a dimer of 3 pairs of non-identical chains Aa (Mr= 66,800), Bb (Mr= 52,000) and g (Mr=46,500) cross-linked by disulfide bonds in their N-terminal segments. The molecule has 2 terminal D domains and one central E domain, all three domains are separated when fibrinogen is degradated by plasmin. MW is 340 kDa. Concentration in human plasma is 2-3 mg/ml.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.