|Tested species reactivity||Human|
|Published species reactivity||Pig, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Highly purified, human fibronectin from plasma.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-23693 detects fibronectin in human samples. This antibody does not cross-react with Fibrinogen or other serum proteins.
PA1-23693 has been successfully used in immunohistochemistry (frozen and FFPE) procedures. Prolonged fixation in buffered formalin may destroy the epitope. Auto/Zyme predigestion is recommended when using this antibody with formalin-fixed paraffin-embedded tissue sections. Suggested possitive controls for this product are human tonsil, liver, skin or kidney.
The PA1-23693 immunogen is highly purified, human fibronectin.
Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and extracellular matrix. Fibronectin is involved in cell adhesion and migration process including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They also occur as dimmer of two 200 kDa subunits. Fibronectin has been implicated in carcinoma development in lung cancer. Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Blood-brain barrier properties in vitro depend on composition and assembly of endogenous extracellular matrices.
PA1-23693 was used in ELISA to learn about dependence on composition and assembly of endogenous extracellular matrices by blood-brain barrier properties in vitro
|Zobel K,Hansen U,Galla HJ||Cell and tissue research (365:233)||2016|
Evaluation of capsular and acapsular strains of S. aureus in an experimental brain abscess model.
PA1-23693 was used in immunohistochemistry to investigate the strain difference between capsular and acapsular Stapylococcus aureus in the pathogenesis of brain abscess
|Esen N,Wagoner G,Philips N||Journal of neuroimmunology (218:83)||2010|
Simvastatin abates development of renal fibrosis in experimental renovascular disease.
PA1-23693 was used in immunohistochemistry to investigate the effect of simvastatin on renal fibrosis
|Chade AR,Zhu XY,Grande JP,Krier JD,Lerman A,Lerman LO||Journal of hypertension (26:1651)||2008|