Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Nordic
Fibronectin Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Product Details
RAHU-FBN
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Human fibonectin
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
0.7mg/mL sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstituted by adding 1 mL sterile distilled water.
The antiSerum does not cross react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a reaction with cow, Cat, Dog and Monkey have been observed.
In immunoelectrophoresis use 2 µL amniotic fluid or equivalent against 120 µL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µL antiserum in 3 mm diameter center well and 2 µL amniotic fluid or hepatoma serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Target Information
Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ferritin L subunit; ferritin L-chain; Fibronectin; FN; Migration stimulating factor (MSF); migration-stimulating factor
Gene Aliases: CIG; ED-B; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF
UniProt ID: (Human) P02751
Entrez Gene ID: (Human) 2335
angiogenesis
cell activation
regulation of protein phosphorylation
platelet degranulation
response to ischemia
acute-phase response
cell-substrate junction assembly
cell adhesion
calcium-independent cell-matrix adhesion
positive regulation of cell proliferation
glial cell migration
regulation of cell shape
response to wounding
response to ozone
positive regulation of gene expression
positive regulation of peptidase activity
peptide cross-linking
extracellular matrix disassembly
extracellular matrix organization
integrin activation
substrate adhesion-dependent cell spreading
cellular response to vascular endothelial growth factor stimulus
endodermal cell differentiation
cellular response to platelet-derived growth factor stimulus
wound healing
negative regulation of apoptotic process
positive regulation of axon extension
positive regulation of fibroblast proliferation
leukocyte migration
positive regulation of chemotaxis
response to glucocorticoid
regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to mercury ion
cellular response to glucose stimulus
cellular response to interleukin-1
cellular response to prostaglandin E stimulus
cellular response to transforming growth factor beta stimulus
cellular response to BMP stimulus
positive regulation of substrate-dependent cell migration, cell attachment to substrate
negative regulation of transforming growth factor-beta secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support