Western blot analysis of Firefly Luciferase using 30µg of A) 293T (B) 293T transfected firefly and C) 293T transfected Renilla Luciferase lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Firefly Luciferase polyclonal antibody (Product # PA5-32209) at a dilution of 1:5000.
|Tested species reactivity||Luciferase|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment contain a sequence corresponding to a region within amino acids 318 and 550 of Firefly Luciferase|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA5-32209 targets Firefly Luciferase in WB applications and shows reactivity with Firefly samples.
The PA5-32209 immunogen is a recombinant fragment corresponding to a region within amino acids 318 and 550 of Firefly Luciferase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Replication-Competent Influenza Virus and Respiratory Syncytial Virus Luciferase Reporter Strains Engineered for Co-Infections Identify Antiviral Compounds in Combination Screens.
PA5-32209 was used in western blot to develop a high-throughput screening protocol for the simultaneous identification of pathogen- and host-targets when cells are infected with influenza A virus and respiratory syncytial virus
|Yan D,Weisshaar M,Lamb K,Chung HK,Lin MZ,Plemper RK||Biochemistry (54:5589)||2015|