Western blot of Flavin reductase in human liver lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with a Flavin reductase polyclonal antibody (Product# PA5-37842) at a dilution of 0.1ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-SRLPSEGPRPAH, from the internal region of BLVRB (aa38-49)|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-0.3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B (EC 220.127.116.11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.